MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Curr Microbiol. 2012 Sep;65(3):244-53. doi: 10.1007/s00284-012-0152-1. Epub 2012 May 27.
A total of 48 water samples were collected from six water treatment plants in Wuhan and analyzed by real-time PCR assay for viral identification of enterovirus (EV), rotavirus group A (RVA), human adenovirus (HAdV) as well as human adenovirus subgroup F (HAdVF) during the period from December 2010 to October 2011. HAdV, HAdVF, and RVA were all positively detected in the samples of source water and treated drinking water. EV could be found in 46 % (11/24) of all the source water samples, but only 21 % (5/24) positive in treated drinking water. The concentrations of these three kinds of enteric viruses detected were as follows: HAdV > RVA > EV. The highest removal rate was EV (97 %), followed by RVA (82 %), HAdV (73 %), and HAdVF (72 %). HAdV and RVA have been abundant in untreated river water and finished water after conventional processes of water treatment plants, while bacterial indicators could not be detected in tap water, which met the standard of China for drinking water bacterial quality. Some factors that could affect the accuracy of qPCR detection are also discussed in this study.
从 2010 年 12 月至 2011 年 10 月，共采集了武汉市 6 家水厂的 48 个水样，采用实时荧光定量 PCR 方法对水样中的肠道病毒（EV）、轮状病毒 A 组（RVA）、人腺病毒（HAdV）以及人腺病毒亚组 F（HAdVF）进行了鉴定。在原水和处理饮用水中均检测到了 HAdV、HAdVF 和 RVA。在所有原水样本中，EV 的检出率为 46%（11/24），而在处理饮用水中仅为 21%（5/24）。这三种肠道病毒的检出浓度为：HAdV>RVA>EV。这三种病毒的去除率最高的是 EV（97%），其次是 RVA（82%）、HAdV（73%）和 HAdVF（72%）。未经处理的河水和经过常规处理工艺的水厂出水均含有丰富的 HAdV 和 RVA，而自来水中则未检测到细菌指标，符合中国饮用水细菌质量标准。本研究还讨论了一些可能影响 qPCR 检测准确性的因素。
