酶联荧光测定法：用于检测人轮状病毒的超灵敏固相测定法。
Enzyme-linked fluorescence assay: Ultrasensitive solid-phase assay for detection of human rotavirus.
作者信息
Yolken R H, Stopa P J
出版信息
J Clin Microbiol. 1979 Sep;10(3):317-21. doi: 10.1128/jcm.10.3.317-321.1979.
Abstract
Enzyme-linked immunosorbent assay (ELISA) has proven to be a useful assay system for the direct detection of infectious agents. However, when the usual color-producing substrates are employed, relatively large amounts of substrate must be hydrolyzed by the bound enzyme before detection can be achieved. We attempted to improve the sensitivity of ELISA by utilizing a substrate that yields a fluorescent product on enzyme action. The enzyme-linked fluorescence assay (ELFA) based on this principle was approximately 100 times more sensitive than the corresponding ELISA or radioimmunoassay for the detection of human rotavirus in a standard stool suspension. In addition, the ELFA for human rotavirus was capable of detecting antigen in six specimens that were negative by ELISA. Five of these specimens were obtained late in the course of confirmed rotavirus infections. ELFA provides a simple, reliable, ultrasensitive method for the rapid detection of viral antigen.
摘要
酶联免疫吸附测定（ELISA）已被证明是一种用于直接检测感染因子的有用检测系统。然而，当使用常规的显色底物时，在实现检测之前，结合的酶必须水解相对大量的底物。我们试图通过利用一种在酶作用下产生荧光产物的底物来提高ELISA的灵敏度。基于这一原理的酶联荧光测定（ELFA）在检测标准粪便悬液中的人轮状病毒时，其灵敏度比相应的ELISA或放射免疫测定高约100倍。此外，用于检测人轮状病毒的ELFA能够检测出6份ELISA检测为阴性的标本中的抗原。其中5份标本是在确诊的轮状病毒感染病程后期获得的。ELFA为快速检测病毒抗原提供了一种简单、可靠、超灵敏的方法。
相似文献
1
Enzyme-linked fluorescence assay: Ultrasensitive solid-phase assay for detection of human rotavirus.酶联荧光测定法：用于检测人轮状病毒的超灵敏固相测定法。
J Clin Microbiol. 1979 Sep;10(3):317-21. doi: 10.1128/jcm.10.3.317-321.1979.
2
Comparison of electron microscopy, enzyme-linked immunosorbent assay, solid-phase radioimmunoassay, and indirect immunofluorescence for detection of human rotavirus antigen in faeces.电子显微镜检查、酶联免疫吸附测定、固相放射免疫测定及间接免疫荧光法检测粪便中人类轮状病毒抗原的比较
J Clin Pathol. 1979 Jul;32(7):700-5. doi: 10.1136/jcp.32.7.700.
3
Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces.用于检测犊牛粪便中轮状病毒抗原的五种诊断方法的比较
Med Microbiol Immunol. 1978 Nov 17;166(1-4):157-63. doi: 10.1007/BF02121145.
4
Analysis of nonspecific reactions in enzyme-linked immunosorbent assay testing for human rotavirus.人轮状病毒酶联免疫吸附测定中非特异性反应的分析
J Clin Microbiol. 1979 Nov;10(5):703-7. doi: 10.1128/jcm.10.5.703-707.1979.
5
Measurement of rotavirus antibody by an enzyme-linked immunosorbent assay blocking assay.通过酶联免疫吸附试验阻断法检测轮状病毒抗体。
J Clin Microbiol. 1978 Sep;8(3):283-7. doi: 10.1128/jcm.8.3.283-287.1978.
6
Comparison of hemagglutination-inhibition, complement-fixation and enzyme-linked immunosorbent assay for quantitation of human rotavirus antibodies.血凝抑制试验、补体结合试验及酶联免疫吸附测定法在定量检测人轮状病毒抗体中的比较
Arch Virol. 1979;62(2):131-6. doi: 10.1007/BF01318065.
7
Enzyme-linked immunosorbent assay for the detection of human rotavirus in stools.用于检测粪便中人类轮状病毒的酶联免疫吸附测定法。
J Virol Methods. 1983 Aug;7(2):65-72. doi: 10.1016/0166-0934(83)90093-9.
8
Comparison of an enzyme-linked immunosorbent assay for quantitation of rotavirus antibodies with complement fixation in an epidemiological survey.在一项流行病学调查中，用于定量检测轮状病毒抗体的酶联免疫吸附测定法与补体结合试验的比较。
J Clin Microbiol. 1978 Sep;8(3):268-76. doi: 10.1128/jcm.8.3.268-276.1978.
9
Use of antisera against bovine (NCDV) and simian (SA11) rotaviruses in ELISA to detect different types of human rotavirus.在酶联免疫吸附测定（ELISA）中使用抗牛（NCDV）和猴（SA11）轮状病毒的抗血清来检测不同类型的人轮状病毒。
Can J Microbiol. 1979 Sep;25(9):1118-24. doi: 10.1139/m79-174.
引用本文的文献
1
Application of Enzyme-Linked Fluorescence Assay (ELFA) to Obtain In Vivo Matured Dog Oocytes through the Assessment of Progesterone Level.酶联荧光分析法（ELFA）在通过评估孕酮水平获取体内成熟犬卵母细胞中的应用。
Animals (Basel). 2023 Jun 5;13(11):1885. doi: 10.3390/ani13111885.
2
Quercetin and Ferulic Acid Elicit Estrogenic Activities In Vivo and In Silico.槲皮素和阿魏酸在体内和体外具有雌激素活性。
Molecules. 2023 Jun 29;28(13):5112. doi: 10.3390/molecules28135112.
3
Enhanced Sensitivity for Detection of HIV-1 p24 Antigen by a Novel Nuclease-Linked Fluorescence Oligonucleotide Assay.通过新型核酸酶连接荧光寡核苷酸分析法提高检测HIV-1 p24抗原的灵敏度
PLoS One. 2015 Apr 27;10(4):e0125701. doi: 10.1371/journal.pone.0125701. eCollection 2015.
4
High Prevalence of Symptomatic Hepatitis A Infection in Rural Area of Chaharmahal VA Bakhtiari Province, Iran.伊朗恰哈马哈勒-巴赫蒂亚里省农村地区有症状甲型肝炎感染的高流行率。
J Clin Diagn Res. 2015 Feb;9(2):DC01-3. doi: 10.7860/JCDR/2015/9798.5515. Epub 2015 Feb 1.
5
Development of a fluorescent enzyme-linked DNA aptamer-magnetic bead sandwich assay and portable fluorometer for sensitive and rapid leishmania detection in sandflies.建立一种荧光酶联 DNA 适体-磁珠夹心检测法和便携式荧光计，用于灵敏、快速地检测沙蝇中的利什曼原虫。
J Fluoresc. 2014 Jan;24(1):267-77. doi: 10.1007/s10895-013-1315-6. Epub 2013 Nov 13.
6
Staphylococcal enterotoxin type A internal deletion mutants: serological activity and induction of T-cell proliferation.A型葡萄球菌肠毒素内部缺失突变体：血清学活性及T细胞增殖诱导
Infect Immun. 1993 May;61(5):2059-68. doi: 10.1128/iai.61.5.2059-2068.1993.
7
Comparison of fluorescent and colorigenic substrates for enzyme immunoassays.酶免疫测定中荧光底物与显色底物的比较。
J Clin Microbiol. 1982 May;15(5):757-60. doi: 10.1128/jcm.15.5.757-760.1982.
8
Enzyme immunoassay for detection of Salmonellae in foods.用于检测食品中沙门氏菌的酶免疫测定法。
Appl Environ Microbiol. 1982 Apr;43(4):877-93. doi: 10.1128/aem.43.4.877-893.1982.
9
Investigation of enzyme immunoassay time courses: development of rapid assay systems.酶免疫测定时间进程的研究：快速检测系统的开发。
J Clin Microbiol. 1981 Apr;13(4):738-41. doi: 10.1128/jcm.13.4.738-741.1981.
10
Enzyme immunoassay for direct detection of influenza type A and adenovirus antigens in clinical specimens.用于直接检测临床标本中甲型流感病毒和腺病毒抗原的酶免疫测定法。
J Clin Microbiol. 1982 Jan;15(1):5-11. doi: 10.1128/jcm.15.1.5-11.1982.
本文引用的文献
1
Kinetic behaviour of calf-intestinal alkaline phosphatase with 4-methylumbelliferyl phosphate.小牛肠碱性磷酸酶与4-甲基伞形酮磷酸酯的动力学行为。
Biochem J. 1965 Oct;97(1):95-103. doi: 10.1042/bj0970095.
2
Studies on the stability of 3-O-methyl fluorescein phosphate.3-O-甲基荧光素磷酸酯的稳定性研究。
Biochim Biophys Acta. 1968 Jun 4;159(2):420-2. doi: 10.1016/0005-2744(68)90096-x.
3
Detection of Australia antigen and antibody by means of radioimmunoassay techniques.采用放射免疫测定技术检测澳大利亚抗原和抗体。
J Infect Dis. 1970 May;121(5):550-4. doi: 10.1093/infdis/121.5.550.
4
Application of immunofluorescent antibody technique in rapid diagnosis of respiratory syncytial virus infection.免疫荧光抗体技术在呼吸道合胞病毒感染快速诊断中的应用。
Br Med J. 1968 Aug 10;3(5614):340-3. doi: 10.1136/bmj.3.5614.340.
5
Letter: Virus-like particles in hepatitis-A-positive faecal extracts.信件：甲型肝炎阳性粪便提取物中的病毒样颗粒。
Lancet. 1974 Nov 2;2(7888):1083-4. doi: 10.1016/s0140-6736(74)92188-6.
6
7
Enzyme immunoassays in diagnostic medicine. Theory and practice.诊断医学中的酶免疫测定。理论与实践。
Bull World Health Organ. 1976;53(1):55-65.
8
Enzyme-immunoassay.酶免疫测定法
Clin Chem. 1976 Aug;22(8):1243-55.
9
Rapid diagnosis of Legionnaires' disease by direct immunofluorescent staining.通过直接免疫荧光染色快速诊断军团病
Ann Intern Med. 1979 Jan;90(1):1-4. doi: 10.7326/0003-4819-90-1-1.
10
Use of a free viral immunofluorescence assay to detect human reovirus-like agent in human stools.使用游离病毒免疫荧光测定法检测人类粪便中的人呼肠孤病毒样因子。
Infect Immun. 1977 May;16(2):467-70. doi: 10.1128/iai.16.2.467-470.1977.
Zotero 插件