Chu Chia-Yu, Peng Fu-Chuo, Chiu Ying-Fang, Lee Hsing-Chuan, Chen Chien-Wen, Wei Jiun-Chiou, Lin Jiang-Jen
Department of Dermatology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
PLoS One. 2012;7(6):e38360. doi: 10.1371/journal.pone.0038360. Epub 2012 Jun 5.
Silver nanoparticles supported on nanoscale silicate platelets (AgNP/NSP) possess interesting properties, including a large surface area and high biocide effectiveness. The nanohybrid of AgNP/NSP at a weight ratio 7/93 contains 5-nm Ag particles supported on the surface of platelets with dimensions of approximately 80×80×1 nm(3). The nanohybrid expresses a trend of lower cytotoxicity at the concentration of 8.75 ppm Ag and low genotoxicity. Compared with conventional silver ions and the organically dispersed AgNPs, the nanohybrid promotes wound healing. We investigated overall wound healing by using acute burn and excision wound healing models. Tests on both infected wound models of mice were compared among the AgNP/NSP, polymer-dispersed AgNPs, the commercially available Aquacel, and silver sulfadiazine. The AgNP/NSP nanohybrid was superior for wound appearance, but had similar wound healing rates, vascular endothelial growth factor (VEGF)-A levels and transforming growth factor (TGF)-β1 expressions to Aquacel and silver sulfadiazine.
负载于纳米级硅酸盐薄片上的银纳米颗粒（AgNP/NSP）具有有趣的特性，包括大表面积和高杀菌效果。重量比为7/93的AgNP/NSP纳米杂化物包含尺寸约为80×80×1 nm(3)的薄片表面负载的5纳米银颗粒。该纳米杂化物在8.75 ppm银浓度下表现出较低细胞毒性和低遗传毒性的趋势。与传统银离子和有机分散的AgNP相比，该纳米杂化物可促进伤口愈合。我们通过急性烧伤和切除伤口愈合模型研究了整体伤口愈合情况。在小鼠的两种感染伤口模型上对AgNP/NSP、聚合物分散的AgNP、市售的Aquacel和磺胺嘧啶银进行了比较测试。AgNP/NSP纳米杂化物在伤口外观方面表现更优，但在伤口愈合率、血管内皮生长因子（VEGF）-A水平和转化生长因子（TGF）-β1表达方面与Aquacel和磺胺嘧啶银相似。
