Connelly Philip W, Maguire Graham F, Nash Michelle M, Rapi Lindita, Yan Andrew T, Prasad G V Ramesh
Department of Medicine, Keenan Research Centre, Li Ka Shing Knowledge Institute, St. Michael's Hospital, University of Toronto, Toronto, ON, Canada M5B 1W8.
J Lipids. 2012;2012:608580. doi: 10.1155/2012/608580. Epub 2012 Jun 3.
South Asian renal transplant recipients have a higher incidence of cardiovascular disease compared with Caucasian renal transplant recipients. We carried out a study to determine whether paraoxonase 1, a novel biomarker for cardiovascular risk, was decreased in South Asian compared with Caucasian renal transplant recipients. Subjects were matched two to one on the basis of age and sex for a total of 129 subjects. Paraoxonase 1 was measured by mass, arylesterase activity, and two-substrate phenotype assay. Comparisons were made by using a matched design. The frequency of PON1 QQ, QR and RR phenotype was 56%, 37%, and 7% for Caucasian subjects versus 35%, 44%, and 21% for South Asian subjects (χ(2) = 7.72, P = 0.02). PON1 mass and arylesterase activity were not significantly different between South Asian and Caucasian subjects. PON1 mass was significantly associated with PON1 phenotype (P = 0.0001), HDL cholesterol (P = 0.009), LDL cholesterol (P = 0.02), and diabetes status (P < 0.05). Arylesterase activity was only associated with HDL cholesterol (P = 0.003). Thus the frequency of the PON1 RR phenotype was higher and that of the QQ phenotype was lower in South Asian versus Caucasian renal transplant recipients. However, ethnicity was not a significant factor as a determinant of PON1 mass or arylesterase activity, with or without analysis including PON1 phenotype. The two-substrate method for determining PON1 phenotype may be of value for future studies of cardiovascular complications in renal transplant recipients.
与白种人肾移植受者相比，南亚肾移植受者患心血管疾病的发生率更高。我们开展了一项研究，以确定心血管疾病风险的新型生物标志物对氧磷酶1在南亚肾移植受者中是否比白种人肾移植受者降低。根据年龄和性别以2比1的比例对受试者进行匹配，共129名受试者。通过质量法、芳基酯酶活性法和双底物表型分析法测定对氧磷酶1。采用匹配设计进行比较。白种人受试者中PON1 QQ、QR和RR表型的频率分别为56%、37%和7%，而南亚受试者中分别为3%、44%和21%（χ(2)=7.72，P=0.02）。南亚和白种人受试者之间的PON1质量和芳基酯酶活性无显著差异。PON1质量与PON1表型（P=0.0001）、高密度脂蛋白胆固醇（P=0.009）、低密度脂蛋白胆固醇（P=0.02）和糖尿病状态（P<0.05）显著相关。芳基酯酶活性仅与高密度脂蛋白胆固醇相关（P=0.003）。因此，与白种人肾移植受者相比，南亚肾移植受者中PON1 RR表型的频率更高，QQ表型的频率更低。然而，无论是否包括PON1表型进行分析，种族都不是决定PON1质量或芳基酯酶活性的重要因素。用于确定PON1表型的双底物法可能对未来肾移植受者心血管并发症的研究有价值。
