在含有 cAMP 和利福霉素的有限营养培养基上生长来治疗糙皮侧耳中的病毒。

Curing viruses in Pleurotus ostreatus by growth on a limited nutrient medium containing cAMP and rifamycin.

机构信息

Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University, 900 Gajwa-Dong, Jinju, Republic of Korea.

出版信息

J Virol Methods. 2012 Oct;185(1):156-9. doi: 10.1016/j.jviromet.2012.06.002. Epub 2012 Jun 13.

DOI:10.1016/j.jviromet.2012.06.002

PMID:22705083

Abstract

Oyster mushroom spherical virus (OMSV) and oyster mushroom isometric virus (OMIV) are the causative agents of a fruiting body deformation disease in the edible mushroom Pleurotus ostreatus. The curing of these mycoviruses was facilitated by a serial transfer of infected mycelia onto a limited nutrient medium containing 1mM of cAMP and 75 μg/ml of rifamycin (cAMP-rifamycin plate). The mycelia were grown on cAMP-rifamycin plates for 5 successive passages. ELISA and RT-PCR showed that the amount of mycoviruses inside the mycelia decreased significantly with increasing numbers of passages. The mycelia became free of viruses after 5 successive passages. Cultivation of the virus-cured mycelia on a mushroom compost medium produced a normal harvest, whereas the spawn infected with viruses failed to produce any fruiting bodies.

摘要

蚝蘑球形病毒（OMSV）和蚝蘑等面体病毒（OMIV）是导致可食用蘑菇糙皮侧耳果实变形的病原体。通过将受感染的菌丝连续转移到含有 1mM cAMP 和 75μg/ml 利福霉素的有限营养培养基上，可以治愈这些真菌病毒（cAMP-利福霉素平板）。菌丝在 cAMP-利福霉素平板上连续传代 5 次。ELISA 和 RT-PCR 表明，随着传代次数的增加，菌丝内真菌病毒的数量显著减少。经过 5 次连续传代后，菌丝中已无病毒。在蘑菇堆肥培养基上培养病毒处理过的菌丝可正常收获，而感染病毒的种菌则未能产生任何子实体。