State Key Laboratory for Protection and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China.
CAS Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650000, China.
Viruses. 2023 Oct 13;15(10):2088. doi: 10.3390/v15102088.
Rhizoctonia solani virus717 (RhsV717) was isolated from the () AG-2 strain Rhs717. This study isolated a virus designated as Rhizoctonia solani partitivirus BS-5 (RsPV-BS5) from the AG-3 strain BS-5, the causal agent of tobacco target spot disease. The virus was identified as a strain of RhsV717. Transmission electron microscopy (TEM) images showed that RsPV-BS5 had virus particles with a diameter of approximately 40 nm. Importantly, it can be horizontally transmitted through hyphal anastomosis and vertically transmitted via sexual basidiospores. Furthermore, this study demonstrated that RsPV-BS5 infection significantly impedes mycelial growth and induces hypovirulence in tobacco leaves. Thus, RsPV-BS5 presents a promising avenue for biocontrolling tobacco target spot disease. Transcriptome analysis unveiled differential expression of four genes related to cell wall-degrading enzymes between two isogenic strains, 06-2-15V and 06-2-15. These findings shed light on the molecular mechanism through which RsPV-BS5 reduces host pathogenicity.
从 Rhizoctonia solani 病毒 717（RhsV717）AG-2 菌株中分离出 Rhizoctonia solani virus717（RhsV717）。本研究从烟草靶斑病的病原菌 AG-3 菌株 BS-5 中分离出一种被命名为 Rhizoctonia solani partitivirus BS-5（RsPV-BS5）的病毒。该病毒被鉴定为 RhsV717 的一个株系。透射电子显微镜（TEM）图像显示，RsPV-BS5 具有直径约为 40nm 的病毒粒子。重要的是，它可以通过菌丝融合进行水平传播，通过有性担孢子进行垂直传播。此外，本研究表明 RsPV-BS5 感染显著抑制了菌丝生长并诱导了烟草叶片的弱毒。因此，RsPV-BS5 为生物防治烟草靶斑病提供了一个有前景的途径。转录组分析揭示了两个同基因株系 06-2-15V 和 06-2-15 之间与细胞壁降解酶相关的四个基因的差异表达。这些发现揭示了 RsPV-BS5 降低宿主致病性的分子机制。
