Department of Neurobiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
Nat Neurosci. 2012 Jun 17;15(7):949-51. doi: 10.1038/nn.3134.
The stability of dendritic spines in the neocortex is profoundly influenced by sensory experience, which determines the magnitude and pattern of neural firing. By optically manipulating the temporal structure of neural activity in vivo using channelrhodopsin-2 and repeatedly imaging dendritic spines along these stimulated neurons over a period of weeks, we show that the specific pattern, rather than the total amount of activity, determines spine stability in awake mice.
树突棘在大脑新皮层中的稳定性受到感觉体验的深刻影响，而感觉体验决定了神经放电的幅度和模式。我们利用通道视紫红质-2（channelrhodopsin-2）在体内对神经活动的时间结构进行光遗传学操作，并在数周时间内对这些被刺激神经元沿线的树突棘进行重复成像，结果表明，在清醒小鼠中，决定树突棘稳定性的是特定的活动模式，而不是活动的总量。
