Harran University Medical School, Department of Pediatrics, Sanliurfa, Turkey.
Indian Pediatr. 2012 Dec;49(12):958-62. doi: 10.1007/s13312-012-0250-y. Epub 2012 Jun 10.
To compare oxidative status, total antioxidant capacity and values of DNA damage in peripheral blood lymphocytes in children exposed to secondhand cigarette smoke with healthy controls.
Analytical, Observational.
54 children without any chronic diseases, attending the healthy child monitoring polyclinic. These comprised 27 children who had been exposed to passive cigarette smoke and 27 children who had not been exposed to cigarette smoke.
Urine cotinine levels by the chemiluminescent technique; DNA damage by alkaline comet assay; and total oxidant status (TOS) using a novel automated measurement method.
The mean urine cotinine, TOS, Oxidative Stress Index (OSI) and DNA damage values of the group exposed to cigarette smoke were determined to be at significantly higher level compared to the group not exposed to cigarette smoke (P<0.001). No statistically significant difference was determined in the TAS level between the two groups (P=0.1)
The results showed that TOS levels, OSI index and DNA damage in peripheral blood lymphocytes were significantly higher in children exposed to secondhand cigarette smoke than in those not exposed to secondhand cigarette smoke.
比较二手烟暴露儿童与健康对照组外周血淋巴细胞氧化状态、总抗氧化能力和 DNA 损伤值。
分析性、观察性。
54 名无任何慢性疾病、在健康儿童监测门诊就诊的儿童。其中 27 名儿童被动吸烟，27 名儿童未接触香烟烟雾。
化学发光法检测尿可替宁水平；碱性彗星试验检测 DNA 损伤；新型自动测量法检测总氧化剂状态（TOS）。
与未接触香烟烟雾的儿童相比，接触香烟烟雾的儿童的尿可替宁、TOS、氧化应激指数（OSI）和 DNA 损伤值明显更高（P<0.001）。两组间 TAS 水平无统计学差异（P=0.1）。
结果表明，二手烟暴露儿童外周血淋巴细胞 TOS 水平、OSI 指数和 DNA 损伤明显高于未暴露于二手烟的儿童。
