Farjam M, Dehdab P, Abbassnia F, Mehrabani D, Tanideh N, Pakbaz S, Imanieh M H
Department of Pharmacology, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran Red Crescent Med J. 2012 Mar;14(3):164-70. Epub 2012 Mar 1.
As a serious neuropsychiatric disease, hepatic encephalopathy (HE) is a clinical condition with several types regarding chronicity and clinical diversity that can develop as a complication of both acute and chronic liver failure. This study evaluates changes in thioacetamide (TAA)-induced acute hepatic encephalopathy (AHE) in rat as an animal model.
Both genders of C57BL6, BALB/C mice and Sprague Dawley rats; (10 animals in each group) were compared for induction of AHE to clarify which animal and gender were appropriate. The animals (10 male rats in each group) were categorized in 4 groups according to the dose of the TAA administered (200, 300 and 400 mg/kg of TAA at 24 h intervals for 4 days). A control group was treated with solvent of TAA which was water (5 ml/kg/day). The behavioral, biochemical markers of hepatic failure and histological aspects of thioacetamide (TAA) induced AHE and the correlation between the clinical severity and liver failure biomarkers were evaluated.
Rat was shown to be an animal model of choice for AHE while the optimum dosage of TAA to induce AHE was 300 mg/kg/day at 24 h intervals for 4 days. The behavioral score was partially correlated with the rising of some biomarkers and pathological findings.
Rat can be introduced as the animal of choice for AHE to study the pathophysiology, pharmacology and the survival rate of disease in liver transplant patients.
作为一种严重的神经精神疾病，肝性脑病（HE）是一种临床病症，根据慢性程度和临床多样性有多种类型，可作为急性和慢性肝衰竭的并发症出现。本研究评估硫代乙酰胺（TAA）诱导的大鼠急性肝性脑病（AHE）作为动物模型的变化。
比较C57BL6、BALB/C小鼠和Sprague Dawley大鼠的雌雄两性（每组10只动物）诱导AHE的情况，以明确哪种动物和性别合适。根据给予的TAA剂量（以24小时间隔给予200、300和400mg/kg的TAA，持续4天）将动物（每组10只雄性大鼠）分为4组。对照组用TAA的溶剂即水（5ml/kg/天）进行处理。评估硫代乙酰胺（TAA）诱导的AHE的行为、肝衰竭生化标志物和组织学方面，以及临床严重程度与肝衰竭生物标志物之间的相关性。
大鼠被证明是AHE的首选动物模型，而诱导AHE的TAA最佳剂量为300mg/kg/天，以24小时间隔持续4天。行为评分与一些生物标志物的升高及病理结果部分相关。
大鼠可作为AHE的首选动物，用于研究肝移植患者疾病的病理生理学、药理学和生存率。
