Clarke Matthew L, Lee Ji Youn, Samarov Daniel V, Allen David W, Litorja Maritoni, Nossal Ralph, Hwang Jeeseong
Biomed Opt Express. 2012 Jun 1;3(6):1291-9. doi: 10.1364/BOE.3.001291. Epub 2012 May 9.
The design and fabrication of custom-tailored microarrays for use as phantoms in the characterization of hyperspectral imaging systems is described. Corresponding analysis methods for biologically relevant samples are also discussed. An image-based phantom design was used to program a microarrayer robot to print prescribed mixtures of dyes onto microscope slides. The resulting arrays were imaged by a hyperspectral imaging microscope. The shape of the spots results in significant scattering signals, which can be used to test image analysis algorithms. Separation of the scattering signals allowed elucidation of individual dye spectra. In addition, spectral fitting of the absorbance spectra of complex dye mixtures was performed in order to determine local dye concentrations. Such microarray phantoms provide a robust testing platform for comparisons of hyperspectral imaging acquisition and analysis methods.
本文描述了定制微阵列的设计与制造，该微阵列用作高光谱成像系统特性表征中的体模。还讨论了针对生物相关样本的相应分析方法。基于图像的体模设计用于对微阵列机器人进行编程，以便将规定的染料混合物打印到显微镜载玻片上。所得阵列由高光谱成像显微镜成像。斑点的形状会产生显著的散射信号，可用于测试图像分析算法。散射信号的分离有助于阐明各个染料的光谱。此外，对复杂染料混合物的吸收光谱进行光谱拟合，以确定局部染料浓度。此类微阵列体模为比较高光谱成像采集和分析方法提供了一个强大的测试平台。
