Clough W
Biochemistry. 1979 Oct 16;18(21):4517-21. doi: 10.1021/bi00588a009.
A deoxyribonuclease activity from Epstein--Barr (EB) virus producer lymphocyte cell lines which is correlated with viral production and which is not present in virus non-producer or negative lymphocyte cell lines has been purified 220-fold with 20% recovery and characterized. This nuclease copurifies through diethylaminoethylcellulose column chromatography with the EB virus induced deoxyribonucleic acid (DNA) polymerase in EB virus producer cells which was recently reported by this laboratory, but elutes as a separate peak of activity upon phosphocellulose chromatography. This nuclease activity has a sedimentation coefficient of 4.0 S, a strong divalent cation requirement, an alkaline pH optimum, and the ability to utilize both native and denatured lymphocyte DNA as substrate, reducing both to monophosphonucleosides.
来自爱泼斯坦 - 巴尔（EB）病毒产生淋巴细胞系的一种脱氧核糖核酸酶活性已被纯化了220倍，回收率为20%，并进行了特性鉴定。该活性与病毒产生相关，且不存在于病毒非产生或阴性淋巴细胞系中。这种核酸酶通过二乙氨基乙基纤维素柱色谱与EB病毒产生细胞中EB病毒诱导的脱氧核糖核酸（DNA）聚合酶共纯化，本实验室最近报道过该聚合酶，但在磷酸纤维素色谱上作为一个单独的活性峰洗脱。这种核酸酶活性的沉降系数为4.0 S，强烈需要二价阳离子，最适pH为碱性，并且能够利用天然和变性的淋巴细胞DNA作为底物，将两者都降解为单磷酸核苷。
