Li Yongqiang, Ma Peixiang, Tao Quan, Krause Hans-Joachim, Yang Siwei, Ding Guqiao, Dong Hui, Xie Xiaoming
State Key Laboratory of Functional Materials of Informatics, Shanghai Institute of Microsystem and Information Technology (SIMIT), Chinese Academy of Sciences, Shanghai, 200050, PR China.
CAS Center for ExcelleNce in Superconducting Electronics (CENSE), Chinese Academy of Sciences, Shanghai, 200050, PR China.
Sens Actuators B Chem. 2021 Jun 15;337:129786. doi: 10.1016/j.snb.2021.129786. Epub 2021 Mar 15.
The rapid and sensitive diagnosis of the highly contagious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is one of the crucial issues at the outbreak of the ongoing global pandemic that has no valid cure. Here, we propose a SARS-CoV-2 antibody conjugated magnetic graphene quantum dots (GQDs)-based magnetic relaxation switch (MRSw) that specifically recognizes the SARS-CoV-2. The probe of MRSw can be directly mixed with the test sample in a fully sealed vial without sample pretreatment, which largely reduces the testers' risk of infection during the operation. The closed-tube one-step strategy to detect SARS-CoV-2 is developed with home-made ultra-low field nuclear magnetic resonance (ULF NMR) relaxometry working at 118 μT. The magnetic GQDs-based probe shows ultra-high sensitivity in the detection of SARS-CoV-2 due to its high magnetic relaxivity, and the limit of detection is optimized to 248 Particles mL. Meanwhile, the detection time in ULF NMR system is only 2 min, which can significantly improve the efficiency of detection. In short, the magnetic GQDs-based MRSw coupled with ULF NMR can realize a rapid, safe, and sensitive detection of SARS-CoV-2.
对高传染性的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）进行快速灵敏的诊断，是当前这场尚无有效治愈方法的全球大流行疫情爆发时的关键问题之一。在此，我们提出一种基于SARS-CoV-2抗体偶联磁性石墨烯量子点（GQDs）的磁弛豫开关（MRSw），它能特异性识别SARS-CoV-2。MRSw的探针可在完全密封的小瓶中直接与测试样品混合，无需样品预处理，这在很大程度上降低了操作人员在操作过程中的感染风险。利用自制的工作在118 μT的超低场核磁共振（ULF NMR）弛豫测量技术，开发了用于检测SARS-CoV-2的封闭管一步法策略。基于磁性GQDs的探针由于其高磁弛豫率，在检测SARS-CoV-2时显示出超高灵敏度，检测限优化至248个颗粒/毫升。同时，ULF NMR系统中的检测时间仅为2分钟，这可显著提高检测效率。简而言之，基于磁性GQDs的MRSw与ULF NMR相结合，可实现对SARS-CoV-2的快速、安全且灵敏的检测。
