Department of Preventive Dentistry, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
Expert Rev Proteomics. 2012 Jun;9(3):311-23. doi: 10.1586/epr.12.16.
This review covers developments in the study of polymicrobial communities, biofilms and selected areas of host response relevant to dental plaque and related areas of oral biology. The emphasis is on recent studies in which proteomic methods, particularly those using mass spectrometry as a readout, have played a major role in the investigation. The last 5-10 years have seen a transition of such methods from the periphery of oral biology to the mainstream, as in other areas of biomedical science. For reasons of focus and space, the authors do not discuss biomarker studies relevant to improved diagnostics for oral health, as this literature is rather substantial in its own right and deserves a separate treatment. Here, global gene regulation studies of plaque-component organisms, biofilm formation, multispecies interactions and host-microbe interactions are discussed. Several aspects of proteomics methodology that are relevant to the studies of multispecies systems are commented upon.
这篇综述涵盖了多微生物群落、生物膜以及与牙菌斑和相关口腔生物学领域相关的宿主反应的研究进展。重点是最近的研究，其中蛋白质组学方法，特别是使用质谱作为读出的方法，在研究中发挥了重要作用。在过去的 5-10 年中，这些方法已经从口腔生物学的边缘领域过渡到主流领域，就像在其他生物医学科学领域一样。由于重点和篇幅的原因，作者没有讨论与口腔健康改进诊断相关的生物标志物研究，因为这方面的文献本身就相当丰富，值得单独处理。在这里，我们讨论了菌斑成分生物、生物膜形成、多物种相互作用和宿主-微生物相互作用的全局基因调控研究。还对与多物种系统研究相关的蛋白质组学方法的几个方面进行了评论。
