National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.
Bioinformatics. 2012 Oct 1;28(19):2407-11. doi: 10.1093/bioinformatics/bts464. Epub 2012 Jul 24.
Gibberellic acids (GAs) are key plant hormones, regulating various aspects of growth and development, which have been at the center of the 'green revolution'. GRAS family proteins, the primary players in GA signaling pathways, remain poorly understood. Using sequence-profile searches, structural comparisons and phylogenetic analysis, we establish that the GRAS family first emerged in bacteria and belongs to the Rossmann fold methyltransferase superfamily. All bacterial and a subset of plant GRAS proteins are likely to function as small-molecule methylases. The remaining plant versions have lost one or more AdoMet (SAM)-binding residues while preserving their substrate-binding residues. We predict that GRAS proteins might either modify or bind small molecules such as GAs or their derivatives.
Supplementary Material for this article is available at Bioinformatics online.
赤霉素（GA）是关键的植物激素，调节生长和发育的各个方面，一直是“绿色革命”的核心。GRAS 家族蛋白是 GA 信号通路的主要参与者，但它们仍未被充分了解。通过序列-轮廓搜索、结构比较和系统发育分析，我们确定 GRAS 家族首先出现在细菌中，属于 Rossmann 折叠甲基转移酶超家族。所有细菌和一部分植物的 GRAS 蛋白可能作为小分子甲基转移酶发挥作用。其余的植物版本丢失了一个或多个 AdoMet（SAM）结合残基，同时保留了其底物结合残基。我们预测 GRAS 蛋白可能修饰或结合小分子，如 GA 或其衍生物。
本文的补充材料可在“Bioinformatics”在线获取。
