Department of Pathology and the Lautenberg Center for General and Tumor Immunology, the Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.
Virus Res. 2012 Oct;169(1):98-106. doi: 10.1016/j.virusres.2012.07.015. Epub 2012 Jul 24.
Cyprinid herpesvirus-3 (CyHV-3) is the cause of a fatal disease in carp and koi fish. The disease is seasonal and appears when water temperatures range from 18 to 28°C. CyHV-3 is a member of the Alloherpesviridae, a family in the Herpesvirales order that encompasses mammalian, avian and reptilian viruses. CyHV-3 is a large double-stranded DNA (dsDNA) herpesvirus with a genome of approximately 295kbp, divergent from other mammalian, avian and reptilian herpesviruses, but bearing several genes similar to cyprinid herpesvirus-1 (CyHV-1), CyHV-2, anguillid herpesvirus-1 (AngHV-1), ictalurid herpesvirus-1 (IcHV-1) and ranid herpes virus-1 (RaHV-1). Here we show that viral DNA synthesis commences 4-8h post-infection (p.i.), and is completely inhibited by pre-treatment with cytosine β-d-arabinofuranoside (Ara-C). Transcription of CyHV-3 genes initiates after infection as early as 1-2h p.i., and precedes viral DNA synthesis. All 156 annotated open reading frames (ORFs) of the CyHV-3 genome are transcribed into RNAs, most of which can be classified into immediate early (IE or α), early (E or β) and late (L or γ) classes, similar to all other herpesviruses. Several ORFs belonging to these groups are clustered along the viral genome.
锦鲤疱疹病毒 3 型（CyHV-3）是引起鲤鱼和锦鲤致命疾病的病原体。该疾病具有季节性，当水温在 18 到 28°C 之间时出现。CyHV-3 是 Alloherpesviridae 家族的一员，该家族属于疱疹病毒目，包含哺乳动物、鸟类和爬行动物的病毒。CyHV-3 是一种大型双链 DNA（dsDNA）疱疹病毒，基因组约为 295kbp，与其他哺乳动物、鸟类和爬行动物疱疹病毒不同，但具有几个与鲤鱼疱疹病毒 1 型（CyHV-1）、CyHV-2、鳗疱疹病毒 1 型（AngHV-1）、美洲牛蛙疱疹病毒 1 型（IcHV-1）和牛蛙疱疹病毒 1 型（RaHV-1）相似的基因。在这里，我们表明病毒 DNA 的合成在感染后 4-8 小时开始，并完全被胞嘧啶 β-D-阿拉伯呋喃糖苷（Ara-C）预处理抑制。CyHV-3 基因的转录在感染后 1-2 小时即可开始，早于病毒 DNA 的合成。CyHV-3 基因组的 156 个注释开放阅读框（ORF）都被转录成 RNA，其中大多数可以分为早期即刻（IE 或α）、早期（E 或β）和晚期（L 或γ）类，与所有其他疱疹病毒相似。属于这些组的几个 ORF 沿着病毒基因组聚集在一起。
