Bielanska Joanna, Hernández-Losa Javier, Moline Teresa, Somoza Rosa, Ramón Y Cajal Santiago, Condom Enric, Ferreres Joan Carles, Felipe Antonio
Molecular Physiology Laboratory, Department of Biochemistry and Molecular Biology, Institute of Biomedicine, University of Barcelona, E-08028 Barcelona.
Department of Pathology, Vall d'Hebron University Hospital, Autonomous University of Barcelona, E-08035 Barcelona.
Oncol Lett. 2012 Aug;4(2):227-230. doi: 10.3892/ol.2012.718. Epub 2012 May 16.
Voltage-dependent K channels (Kv) are involved in the proliferation and differentiation of mammalian cells, since Kv antagonists impair cell cycle progression. Although myofibers are terminally differentiated, some myoblasts may re-enter the cell cycle and proliferate. Since Kv1.3 and Kv1.5 expression is remodeled during tumorigenesis and is involved in smooth muscle proliferation, the purpose of this study was to analyze the expression of Kv1.3 and Kv1.5 in smooth muscle neoplasms. In the present study, we examined human samples of smooth muscle tumors together with healthy specimens. Thus, leiomyoma (LM) and leiomyosarcoma (LMS) tumors were analyzed. Results showed that Kv1.3 was poorly expressed in the healthy muscle and indolent LM specimens, whereas aggressive LMS showed high levels of Kv1.3 expression. Kv1.5 staining was correlated with malignancy. The findings show a remodeling of Kv1.3 and Kv1.5 in human smooth muscle sarcoma. A correlation of Kv1.3 and Kv1.5 expression with tumor aggressiveness was observed. Thus, our results indicate Kv1.5 and Kv1.3 as potential tumorigenic targets for aggressive human LMS.
电压依赖性钾通道（Kv）参与哺乳动物细胞的增殖和分化，因为Kv拮抗剂会损害细胞周期进程。尽管肌纤维是终末分化的，但一些成肌细胞可能重新进入细胞周期并增殖。由于Kv1.3和Kv1.5的表达在肿瘤发生过程中会发生重塑，且参与平滑肌增殖，本研究的目的是分析Kv1.3和Kv1.5在平滑肌肿瘤中的表达。在本研究中，我们检查了人类平滑肌肿瘤样本以及健康标本。因此，对平滑肌瘤（LM）和平滑肌肉瘤（LMS）肿瘤进行了分析。结果显示，Kv1.3在健康肌肉和惰性LM标本中表达较低，而侵袭性LMS显示出高水平的Kv1.3表达。Kv1.5染色与恶性程度相关。这些发现表明人类平滑肌肉瘤中Kv1.3和Kv1.5发生了重塑。观察到Kv1.3和Kv1.5表达与肿瘤侵袭性相关。因此，我们的结果表明Kv1.5和Kv1.3是侵袭性人类LMS潜在的致瘤靶点。
