Barutcigil C, Arslan H, Ozcan E, Harorlı Ot
Department of Restorative Dentistry, Akdeniz University 07058 Antalya, Turkey.
J Conserv Dent. 2012 Jul;15(3):242-5. doi: 10.4103/0972-0707.97947.
The aim of this study was to evaluate the influence of different concentrations of Ethylenediaminetetraacetic acid (EDTA) solution on adhesion, that is, the bond strength of the different adhesive systems, to the pulp chamber dentin.
Recently extracted, sound, human, third molars were cut horizontally to expose the pulp horn. The roof of the pulp chamber and pulp tissue was removed. The teeth were then divided into five main groups. The teeth in each group were treated as follows: group 1, irrigated with saline; group 2, with 5% EDTA for 5 minutes; group 3, with 15% EDTA for 5 minutes; group 4, with 17% EDTA for 5 minutes and group 5, with 19% EDTA for 5 minutes. Treated specimens were dried and divided into 2 subgroups for adhesives; bonded with a total-etching adhesive (Adper Scotchbond Multi-purpose - ASB) or a one bottle of self-etch adhesive system (Clearfil S3 Bond - CS3). After the bonding procedure and composite restoration, teeth were sectioned and dentin sticks were obtained from each group for micro tensile testing (n = 10). Micro tensile testing was performed and scanning electron microscope (SEM) photographs were taken for each irrigated group.
In the ASB group, saline showed statistically higher bond strength values at the different concentrations of EDTA, while the micro-tensile bond strengths of the different concentrations of EDTA were not statistically different. In the CS3 group, saline and 5% EDTA showed statistically higher bond strength values than 17% and 19% EDTA, while the micro-tensile bond strengths of 15% EDTA compared to saline and 5% EDTA and 15% EDTA compared to 17% EDTA and 19% EDTA, were not statistically different.
This study showed that EDTA irrigation can affect the bond strength of adhesive systems on pulp chamber lateral walls. Clinically, low EDTA concentrations can be recommended if self-etch adhesives have been selected.
本研究旨在评估不同浓度的乙二胺四乙酸（EDTA）溶液对不同粘结系统与髓腔牙本质之间粘结力（即粘结强度）的影响。
选取近期拔除的、完好的人第三磨牙，水平切割以暴露髓角。去除髓腔顶部和牙髓组织。然后将牙齿分为五个主要组。每组牙齿的处理如下：第1组，用生理盐水冲洗；第2组，用5% EDTA冲洗5分钟；第3组，用15% EDTA冲洗5分钟；第4组，用17% EDTA冲洗5分钟；第5组，用19% EDTA冲洗5分钟。处理后的标本干燥后再分为两个粘结剂亚组；分别用全酸蚀粘结剂（Adper Scotchbond多用途粘结剂 - ASB）或单瓶自酸蚀粘结系统（Clearfil S3 Bond - CS3）进行粘结。粘结操作和复合树脂修复完成后，将牙齿切片，从每组获取牙本质棒进行微拉伸测试（n = 10）。对每个冲洗组进行微拉伸测试并拍摄扫描电子显微镜（SEM）照片。
在ASB组中，在不同浓度的EDTA下，生理盐水显示出统计学上更高的粘结强度值，而不同浓度的EDTA的微拉伸粘结强度在统计学上无差异。在CS3组中，生理盐水和5% EDTA显示出统计学上高于17%和19% EDTA的粘结强度值，而15% EDTA与生理盐水和5% EDTA相比以及15% EDTA与17%和19% EDTA相比的微拉伸粘结强度在统计学上无差异。
本研究表明，EDTA冲洗会影响粘结系统与髓腔侧壁之间的粘结强度。临床上，如果选择了自酸蚀粘结剂，建议使用低浓度的EDTA。
