Graduate Institute of Immunology, National Taiwan University College of Medicine, Taipei, Taiwan, ROC.
Viral Immunol. 2012 Aug;25(4):277-88. doi: 10.1089/vim.2011.0099.
Severe acute respiratory syndrome (SARS) is characterized by acute respiratory distress syndrome (ARDS) and pulmonary fibrosis, and monocytes/macrophages are the key players in the pathogenesis of SARS. In this study, we compared the transcriptional profiles of SARS coronavirus (SARS-CoV)-infected monocytic cells against that infected by coronavirus 229E (CoV-229E). Total RNA was extracted from infected DC-SIGN-transfected monocytes (THP-1-DC-SIGN) at 6 and 24 h after infection, and the gene expression was profiled in oligonucleotide-based microarrays. Analysis of immune-related gene expression profiles showed that at 24 h after SARS-CoV infection: (1) IFN-α/β-inducible and cathepsin/proteasome genes were downregulated; (2) hypoxia/hyperoxia-related genes were upregulated; and (3) TLR/TLR-signaling, cytokine/cytokine receptor-related, chemokine/chemokine receptor-related, lysosome-related, MHC/chaperon-related, and fibrosis-related genes were differentially regulated. These results elucidate that SARS-CoV infection regulates immune-related genes in monocytes/macrophages, which may be important to the pathogenesis of SARS.
严重急性呼吸综合征（SARS）的特征为急性呼吸窘迫综合征（ARDS）和肺纤维化，而单核细胞/巨噬细胞是 SARS 发病机制中的关键因素。在这项研究中，我们比较了 SARS 冠状病毒（SARS-CoV）感染的单核细胞与冠状病毒 229E（CoV-229E）感染的转录谱。在感染后的 6 和 24 小时，从感染的 DC-SIGN 转染的单核细胞（THP-1-DC-SIGN）中提取总 RNA，并在基于寡核苷酸的微阵列上对基因表达进行分析。免疫相关基因表达谱的分析表明，在 SARS-CoV 感染后 24 小时：（1）IFN-α/β诱导和组织蛋白酶/蛋白酶体基因下调；（2）缺氧/高氧相关基因上调；（3）TLR/TLR 信号、细胞因子/细胞因子受体、趋化因子/趋化因子受体、溶酶体、MHC/伴侣相关和纤维化相关基因的差异调节。这些结果阐明了 SARS-CoV 感染调节单核细胞/巨噬细胞中的免疫相关基因，这可能对 SARS 的发病机制很重要。
