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Raman spectroscopy provides a powerful, rapid diagnostic tool for the detection of tuberculous meningitis in ex vivo cerebrospinal fluid samples.拉曼光谱为检测体外脑脊液样本中的结核性脑膜炎提供了一种强大、快速的诊断工具。
J Biophotonics. 2013 Aug;6(8):567-72. doi: 10.1002/jbio.201200110. Epub 2012 Aug 6.
2
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Diagnostic accuracy of droplet digital PCR analysis of cerebrospinal fluid for tuberculous meningitis in adult patients.基于液滴数字 PCR 分析的脑脊液检测对成人结核性脑膜炎的诊断准确性。
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Detection of 65 kD heat shock protein in cerebrospinal fluid of tuberculous meningitis patients.结核性脑膜炎患者脑脊液中65kD热休克蛋白的检测
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Rapid diagnosis of tuberculous meningitis with a dot enzyme immunoassay to detect antibody in cerebrospinal fluid.采用斑点酶免疫测定法检测脑脊液中的抗体以快速诊断结核性脑膜炎。
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Circulating Mycobacterium bovis peptides and host response proteins as biomarkers for unambiguous detection of subclinical infection.循环牛分枝杆菌肽和宿主反应蛋白作为明确检测亚临床感染的生物标志物。
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本文引用的文献

1
Raman spectroscopy provides a powerful diagnostic tool for accurate determination of albumin glycation.拉曼光谱为准确测定白蛋白糖化提供了一种强大的诊断工具。
PLoS One. 2012;7(2):e32406. doi: 10.1371/journal.pone.0032406. Epub 2012 Feb 29.
2
Incorporation of support vector machines in the LIBS toolbox for sensitive and robust classification amidst unexpected sample and system variability.将支持向量机纳入 LIBS 工具箱中，以实现对意外样本和系统变化具有敏感性和鲁棒性的分类。
Anal Chem. 2012 Mar 20;84(6):2686-94. doi: 10.1021/ac202755e. Epub 2012 Mar 2.
3
Laser-induced breakdown spectroscopy-based investigation and classification of pharmaceutical tablets using multivariate chemometric analysis.基于激光诱导击穿光谱的多元化学计量分析对药物片剂的研究与分类。
Talanta. 2011 Dec 15;87:53-9. doi: 10.1016/j.talanta.2011.09.040. Epub 2011 Oct 1.
4
Raman spectroscopy: a real-time tool for identifying microcalcifications during stereotactic breast core needle biopsies.拉曼光谱法：一种在立体定向乳腺粗针活检过程中识别微钙化的实时工具。
Biomed Opt Express. 2011 Oct 1;2(10):2792-803. doi: 10.1364/BOE.2.002792. Epub 2011 Sep 14.
5
Combined confocal Raman and quantitative phase microscopy system for biomedical diagnosis.用于生物医学诊断的共聚焦拉曼和定量相显微镜联合系统。
Biomed Opt Express. 2011 Sep 1;2(9):2484-92. doi: 10.1364/BOE.2.002484. Epub 2011 Aug 1.
6
Investigation of the specificity of Raman spectroscopy in non-invasive blood glucose measurements.拉曼光谱在无创血糖测量中的特异性研究。
Anal Bioanal Chem. 2011 Jul;400(9):2871-80. doi: 10.1007/s00216-011-5004-5. Epub 2011 Apr 21.
7
Direct analysis of clinical relevant single bacterial cells from cerebrospinal fluid during bacterial meningitis by means of micro-Raman spectroscopy.通过显微拉曼光谱法对细菌性脑膜炎患者脑脊液中临床相关的单个细菌细胞进行直接分析。
J Biophotonics. 2009 Feb;2(1-2):70-80. doi: 10.1002/jbio.200810068.
8
Minimal invasive gender determination of birds by means of UV-resonance Raman spectroscopy.通过紫外共振拉曼光谱法对鸟类进行微创性别鉴定。
Anal Chem. 2008 Feb 15;80(4):1080-6. doi: 10.1021/ac702043q. Epub 2008 Jan 16.
9
Rapid identification of mycobacteria by Raman spectroscopy.通过拉曼光谱法快速鉴定分枝杆菌。
J Clin Microbiol. 2008 Mar;46(3):961-5. doi: 10.1128/JCM.01763-07. Epub 2008 Jan 3.
10
Comparative evaluation of BACTEC 460TB system and Lowenstein-Jensen medium for the isolation of M. tuberculosis from cerebrospinal fluid samples of tuberculous meningitis patients.BACTEC 460TB系统与罗-琴培养基对结核性脑膜炎患者脑脊液样本中结核分枝杆菌分离的比较评估
Indian J Med Microbiol. 2007 Jul;25(3):236-40. doi: 10.4103/0255-0857.34765.

拉曼光谱为检测体外脑脊液样本中的结核性脑膜炎提供了一种强大、快速的诊断工具。

Raman spectroscopy provides a powerful, rapid diagnostic tool for the detection of tuberculous meningitis in ex vivo cerebrospinal fluid samples.

出版信息

J Biophotonics. 2013 Aug;6(8):567-72. doi: 10.1002/jbio.201200110. Epub 2012 Aug 6.

DOI:10.1002/jbio.201200110
PMID:22887773
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4094343/
Abstract

In this letter, we propose a novel method for diagnosis of tuberculous meningitis using Raman spectroscopy. The silicate Raman signature obtained from Mycobacterium tuberculosis positive cases enables specific and sensitive detection of tuberculous meningitis from acquired cerebrospinal fluid samples. The association of silicates with the tuberculosis mycobacterium is discussed. We envision that this new method will facilitate rapid diagnosis of tuberculous meningitis without application of exogenous reagents or dyes and can be aptly used as a complementary screening tool to the existing gold standard methods.

摘要

在这封信中，我们提出了一种使用拉曼光谱诊断结核性脑膜炎的新方法。从分枝杆菌阳性病例中获得的硅酸盐拉曼特征可从获得的脑脊液样本中特异性和敏感地检测结核性脑膜炎。讨论了硅酸盐与结核分枝杆菌的关联。我们设想这种新方法将有助于快速诊断结核性脑膜炎，而无需应用外源性试剂或染料，并且可以作为现有金标准方法的补充筛选工具。