Department of Entomology and Center for Vector Biology and Zoonotic Diseases, The Connecticut Agricultural Experiment Station, New Haven, CT 06504-1106, USA.
Am J Trop Med Hyg. 2012 Oct;87(4):754-9. doi: 10.4269/ajtmh.2012.12-0294. Epub 2012 Aug 13.
A total of 30 Powassan virus (POWV) isolates from Ixodes scapularis collected from Bridgeport and North Branford, CT in 2008, 2010, 2011, and 2012 and one earlier isolate from Ixodes cookei collected in Old Lyme, CT in 1978 were characterized by phylogenetic analysis of their envelope gene sequences. Powassan virus sequences segregated into two major groups termed the deer tick virus (DTV) and Powassan (POW) lineages. The lineage from I. cookei was POW. The remaining viruses from I. scapularis grouped with the DTV lineage. Powassan viruses from Bridgeport were nearly identical and clustered with a virus strain from a human in New York. Viruses from North Branford were homogeneous and grouped with viruses from Massachusetts, northwestern Connecticut, and Ontario. These findings suggest that POWV was independently introduced into these geographical locations in Connecticut and maintained focally in their respective environments. An improved method of isolation of POWV in vitro is described.
总共从 2008 年、2010 年、2011 年和 2012 年在康涅狄格州布里奇波特和北布兰福德收集的 30 株伊蚊扇头蜱分离株和 1978 年在康涅狄格州老莱姆采集的伊蚊库氏分离株的包膜基因序列的系统发育分析，对分离的 30 株 Powassan 病毒（POWV）进行了鉴定。POWV 序列分为两个主要组，称为鹿蜱病毒（DTV）和 Powassan（POW）谱系。来自伊蚊库氏的谱系为 POW。来自伊蚊扇头蜱的其余病毒与 DTV 谱系聚集在一起。来自布里奇波特的 Powassan 病毒几乎相同，并与来自纽约的人类病毒株聚类。来自北布兰福德的病毒是同质的，与来自马萨诸塞州、康涅狄格州西北部和安大略省的病毒聚集在一起。这些发现表明，POWV 是在康涅狄格州的这些地理位置上独立引入的，并在各自的环境中局部存在。描述了一种改进的体外分离 POWV 的方法。
