Department of Toxicology and Pharmacology, School of Pharmacy, Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Neurochem Res. 2012 Dec;37(12):2725-30. doi: 10.1007/s11064-012-0863-5. Epub 2012 Aug 14.
Drug-resistance and adverse effects of current drugs are the most obstacles in the treatment of epilepsy. In a plan for finding new natural anticonvulsant agents, we studied the anticonvulsant effects of essential oil (ZMEO) and methanolic extract (ZMME) of Zhumeria majdae in pentylene tetrazol (PTZ) and maximal electro-shock (MES) models in mice. Mice received different doses of ZMEO and ZMME, 30 min before induction of chemical and electrical convulsions. Neurotoxicity (movement toxicity and sedation) was evaluated using rota-rod test. The mortality was determined after 24 h following injection of different doses of the ZMEO and ZMME. The obtained results show that ZMEO dose-dependently protected mice from tonic convulsions induced by PTZ and MES with effective doses (ED(50)) of 0.26 (0.13-0.39) and 0.27 (0.17-0.37) ml/kg respectively. Toxic doses (TD(50)) in rota-rod test for ZMEO was 0.55 (0.42-0.70) ml/kg. ZMME at dose of 2 g/kg decreased tonic convulsions as much as 40 %. For ZMEO, TD(50) of 0.55 (0.45-0.69) ml/kg was obtained. ZMME significantly decreased the walking time in rota-rod test at dose of 2 g/kg. Lethal dose (LD(50)) of ZMEO was determined as 2.35 (1.98-2.65) ml/kg. ZMME showed about 34 % death of the animals at dose 5 g/kg. The essential oil of Z. majdae could be a good candidate for further anticonvulsive studies.
目前药物的耐药性和不良反应是癫痫治疗中最主要的障碍。在寻找新的天然抗惊厥药物的计划中，我们研究了榆叶梅花（ZMEO）和榆叶梅甲醇提取物（ZMME）在戊四氮（PTZ）和最大电休克（MES）模型中对小鼠的抗惊厥作用。小鼠在化学和电惊厥诱导前 30 分钟接受不同剂量的 ZMEO 和 ZMME。通过转棒试验评估神经毒性（运动毒性和镇静）。注射不同剂量的 ZMEO 和 ZMME 24 小时后，确定死亡率。结果表明，ZMEO 剂量依赖性地保护小鼠免受 PTZ 和 MES 诱导的强直发作，有效剂量（ED（50））分别为 0.26（0.13-0.39）和 0.27（0.17-0.37）ml/kg。ZMEO 在转棒试验中的毒性剂量（TD（50））为 0.55（0.42-0.70）ml/kg。ZMME 剂量为 2 g/kg 可使强直发作减少 40%。对于 ZMEO，TD（50）为 0.55（0.45-0.69）ml/kg。ZMME 在 2 g/kg 剂量下显著减少转棒试验中的行走时间。ZMEO 的致死剂量（LD（50））为 2.35（1.98-2.65）ml/kg。ZMME 在 5 g/kg 剂量下显示约 34%的动物死亡。榆叶梅花的精油可能是进一步抗惊厥研究的良好候选物。
