Istituto di Fisica Applicata Nello Carrara, Consiglio Nazionale delle Ricerche, Sesto Fiorentino, Italy.
J Biophotonics. 2012 Nov;5(11-12):868-77. doi: 10.1002/jbio.201200115. Epub 2012 Aug 17.
We report new advancements in the biomedical exploitation of plasmonic nanoparticles as an effective platform for the photothermal repair of biological tissue. Chitosan films are loaded with gold nanorods with intense optical absorption in the "therapeutic window" of deepest light penetration through the body, and then activated by near infrared laser excitation to give adhesion with adjacent connective tissues. The adhesion consists of 0.07 mm(2) welds of ~20 kPa tensile strength at the film/tissue interface, which are obtained by administration of pulses with duration in the hundreds of millisecond timescale from a diode laser at ~130 J cm(-2). We investigate the adhesive effect as a function of pulse power and duration and identify an optimal operative window to achieve effective and reproducible welds with minimal detrimental superheating. These results may prove valuable to standardize laser bonding techniques and meet current needs for new knowledge which is urged by the penetration of nanotechnology into biomedical optics.
我们报告了在生物医学利用等离子体纳米粒子方面的新进展，将其作为光热修复生物组织的有效平台。壳聚糖薄膜中负载有金纳米棒，其在身体最深部光穿透的“治疗窗口”中具有强烈的光吸收，然后通过近红外激光激发使其与相邻的结缔组织结合。这种结合是通过在几百毫秒时间尺度内从二极管激光以 130 J cm(-2) 的强度施加脉冲来实现的，在薄膜/组织界面处形成了 0.07 mm(2) 的焊接，其拉伸强度约为 20 kPa。我们研究了作为脉冲功率和持续时间的函数的粘附效果，并确定了一个最佳的操作窗口，以实现具有最小过热危害的有效和可重复的焊接。这些结果可能对标准化激光键合技术有价值，并满足当前对纳米技术渗透到生物医学光学领域所急需的新知识的需求。
