Faculty of Veterinary Medicine, Institute of Physiological Chemistry, University of Leipzig, An den Tierkliniken 1, 04103 Leipzig, Germany.
Curr Microbiol. 2012 Dec;65(6):649-55. doi: 10.1007/s00284-012-0207-3. Epub 2012 Aug 18.
In the present study, using the murine monocyte/macrophage cell line RAW264.7 as a model system, we analyzed the phagocytosis rate and the bactericidal capacity of polyunsaturated fatty acids (PUFA)-enriched macrophages against Pseudomonas aeruginosa and Rhodococcus equi. The P. aeruginosa strain ATCC 10145, the virulent R. equi strain ATCC 33701, and the non-virulent R. equi strain ATCC 6939 were examined. Flow cytometric detection of intracellular microorganisms in combination with viability assays were used to determine the impact of PUFA on the number of engulfed, surviving as well as replicating bacteria. Macrophage enrichment with PUFA resulted in an increase of the internalization rate of the microorganisms by the immune cells. Moreover, an impeding action of the unsaturated fatty acids on the intracellular survival rates of the virulent strains P. aeruginosa ATCC 10145 and R. equi ATCC 33701 could be observed. The n-3 fatty acid docosahexaenoic acid (DHA) as well as the n-6 fatty acid arachidonic acid (AA) showed the most pronounced effects. Taken together, our data support the idea of supplementing PUFA to immunocompromised individuals as well as to people suffering from chronic infections with P. aeruginosa or R. equi to improve macrophage phagocytic and microbicidal activity.
在本研究中，我们使用小鼠单核/巨噬细胞系 RAW264.7 作为模型系统，分析了富含多不饱和脂肪酸 (PUFA) 的巨噬细胞对铜绿假单胞菌和马红球菌的吞噬率和杀菌能力。我们检测了铜绿假单胞菌菌株 ATCC 10145、毒力马红球菌菌株 ATCC 33701 和非毒力马红球菌菌株 ATCC 6939。通过流式细胞术检测细胞内微生物结合活力测定来确定 PUFA 对吞噬、存活和复制细菌数量的影响。PUFA 对巨噬细胞的富集导致免疫细胞对微生物的内化率增加。此外，还观察到不饱和脂肪酸对毒力菌株铜绿假单胞菌 ATCC 10145 和马红球菌 ATCC 33701 的细胞内存活率有阻碍作用。n-3 脂肪酸二十二碳六烯酸 (DHA) 和 n-6 脂肪酸花生四烯酸 (AA) 表现出最显著的效果。总之，我们的数据支持向免疫功能低下的个体以及患有铜绿假单胞菌或马红球菌慢性感染的人补充 PUFA 的想法，以提高巨噬细胞的吞噬和杀菌活性。
