Massaioli N, Mainardi S M, Paron L, Redivo L, Rota Scalabrini D, Poccardi G
IV Divisione Universitaria Chirurgia Generale, Ospedale Molinette di Torino.
Ann Ital Chir. 1990 May-Jun;61(3):273-5; discussion 275-6.
The spleen is a peripheral lymphatic organ where lymphocytes stop for long time during their circulation. We studied the peripheral blood lymphocyte subsets both in 30 subjects splenectomized for trauma and in 30 healthy, non splenectomized, subjects. The phenotypical characterization of lymphocyte subpopulations was performed employing monoclonal antibodies by direct immunofluorescence assays with single and double labelling. Comparing the results, we put in evidence, in splenectomized patients, an increase in all the lymphocyte subsets but one (L. G.L. Leu7+). The CD8+ population showed the major increase according with its large representation in the splenic tissue. Splenectomy induces a change in lymphocyte recirculating pool because of the loss of an important anatomical site of migration. This reduction of lymphocyte recirculating capacity can be related to a decreased efficiency in immunocompetence. In fact, many Authors showed that splenectomy is associated with several anomalies of both humoral and cellular immune response. In contrast with this, our group of splenectomized patients doesn't reveal a greater incidence of infections. We conclude that splenectomy realizes a new anatomical situation where the reduction of lymphocyte recirculating capacity can be related to a decreased statistical efficiency in immunocompetence.
脾脏是一个外周淋巴器官，淋巴细胞在其循环过程中会在此长时间停留。我们研究了30例因外伤行脾切除术的患者以及30例健康的未行脾切除术的受试者的外周血淋巴细胞亚群。采用单克隆抗体通过直接免疫荧光法进行单标记和双标记，对淋巴细胞亚群进行表型特征分析。比较结果发现，在脾切除术后的患者中，除一个亚群（L.G.L.Leu7+）外，所有淋巴细胞亚群均有增加。CD8+群体增加最为明显，这与其在脾组织中的大量存在相符。脾切除术导致淋巴细胞再循环池发生变化，因为失去了一个重要的迁移解剖部位。淋巴细胞再循环能力的这种降低可能与免疫能力效率的降低有关。事实上，许多作者表明脾切除术与体液免疫和细胞免疫反应的多种异常有关。与此相反，我们的脾切除患者组并未显示出更高的感染发生率。我们得出结论，脾切除术实现了一种新的解剖学状态，其中淋巴细胞再循环能力的降低可能与免疫能力的统计学效率降低有关。
