利用氟纳米颗粒报告基因和新型血流增强饱和恢复序列快速定量血流中的氧张力。
Rapid quantification of oxygen tension in blood flow with a fluorine nanoparticle reporter and a novel blood flow-enhanced-saturation-recovery sequence.
机构信息
Department of Physics, Washington University, St. Louis, Missouri, USA.
出版信息
Magn Reson Med. 2013 Jul;70(1):176-83. doi: 10.1002/mrm.24436. Epub 2012 Aug 22.
Abstract
We present a novel blood flow-enhanced-saturation-recovery (BESR) sequence, which allows rapid in vivo T1 measurement of blood for both (1)H and (19)F nuclei. BESR sequence is achieved by combining homogeneous spin preparation and time-of-flight image acquisition and therefore preserves high time efficiency and signal-to-noise ratio for (19)F imaging of circulating perfluorocarbon nanoparticles comprising a perfluoro-15-crown-5-ether core and a lipid monolayer (nominal size = 250 nm). The consistency and accuracy of the BESR sequence for measuring T1 of blood was validated experimentally. With a confirmed linear response feature of (19)F R1 with oxygen tension in both salt solution and blood sample, we demonstrated the feasibility of the BESR sequence to quantitatively determine the oxygen tension within mouse left and right ventricles under both normoxia and hyperoxia conditions. Thus, (19)F BESR MRI of circulating perfluorocarbon nanoparticles represents a new approach to noninvasively evaluate intravascular oxygen tension.
摘要
我们提出了一种新颖的血流增强饱和恢复（BESR）序列，可实现血液中 (1)H 和 (19)F 核的快速体内 T1 测量。BESR 序列通过组合均匀的自旋制备和飞行时间图像采集来实现，因此保留了用于循环全氟碳纳米粒子的 (19)F 成像的高时间效率和信噪比，这些纳米粒子包含全氟-15-冠-5-醚核和脂质单层（标称尺寸= 250nm）。BESR 序列测量血液 T1 的一致性和准确性已通过实验得到验证。通过在盐溶液和血液样本中证实 (19)F R1 与氧分压的线性响应特征，我们证明了 BESR 序列在正常氧和高氧条件下定量确定小鼠左右心室中氧分压的可行性。因此，循环全氟碳纳米粒子的 (19)F BESR MRI 代表了一种非侵入性评估血管内氧分压的新方法。
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