Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan, ROC.
Br J Nutr. 2013 May 28;109(10):1779-88. doi: 10.1017/S0007114512003777. Epub 2012 Sep 6.
The present study evaluated the prebiotic effect of a standardised aqueous extract of Anoectochilus formosanus (SAEAF) and its effects on osteoporosis in ovariectomised (OVX) rats. The OVX rats were randomly divided into five groups and orally treated with water, SAEAF (200 and 400 mg/kg daily) and inulin (400 mg/kg daily) for 12 weeks. The sham group was orally treated with water. The SAEAF treatment enhanced the number of faecal bifidobacteria in OVX rats. The results of a Ca-balance experiment showed that SAEAF increased apparent Ca absorption and retention. The OVX rats were killed after SAEAF treatment lasting 12 weeks. The SAEAF decreased the caecal pH values and increased the caecal wall weight, caecal mucosa calbindin-D9k mRNA expression, free-Ca concentration and levels of SCFA in the caecum. The mineral content, density and biomechanical strength of bones were lower in OVX rats than the sham group, but these bone losses were prevented by SAEAF administration. Microtomography scanning showed that the SAEAF-treated rats had higher trabecular bone volume than the OVX rats. These results suggest that SAEAF prevented bone loss associated with ovarian hormone deficiency in the rats.
本研究评估了一种标准化的金线莲（Anoectochilus formosanus）水提物（SAEAF）的益生元作用及其对去卵巢（OVX）大鼠骨质疏松症的影响。将 OVX 大鼠随机分为五组，分别给予水、SAEAF（200 和 400 mg/kg 每日）和菊粉（400 mg/kg 每日）口服治疗 12 周。假手术组给予水口服治疗。SAEAF 处理增加了 OVX 大鼠粪便双歧杆菌的数量。钙平衡实验的结果表明，SAEAF 增加了表观钙吸收和保留。SAEAF 治疗 12 周后处死大鼠。SAEAF 降低了盲肠 pH 值，增加了盲肠壁重量、盲肠黏膜钙结合蛋白-D9k mRNA 表达、游离钙浓度和盲肠中 SCFA 水平。OVX 大鼠的骨矿物质含量、密度和生物力学强度低于假手术组，但 SAEAF 给药可预防这些骨丢失。微断层扫描显示，SAEAF 处理的大鼠的小梁骨体积高于 OVX 大鼠。这些结果表明，SAEAF 可预防与卵巢激素缺乏相关的大鼠骨丢失。
