Kahn D, Gavaler J S, Lai H, Makowka L, Eagon P K, Francavilla A, Starzl T E, Van Thiel D H
Department of Surgery, University of Pittsburgh School of Medicine, Pennsylvania 15261.
Dig Dis Sci. 1990 Jan;35(1):6-11. doi: 10.1007/BF01537215.
Estrogen and androgen receptors within the liver have been reported to modulate the hepatic regenerative response to partial hepatectomy. Moreover, cyclosporine has several untoward effects that might occur as a consequence of alterations in sex hormone activity. To evaluate these questions the following experiments were performed. Estrogen and androgen receptors in cytosol were quantitated in livers of rats treated with cyclosporine or olive oil vehicle before and after partial hepatectomy or a sham operation. Ornithine decarboxylase activity and thymidine kinase activity were assessed as indices of hepatic regeneration. Preoperative levels of estrogen receptor activity in the hepatic cytosol were significantly greater in rats treated with cyclosporine as compared to vehicle treated controls (P less than 0.01). In contrast, preoperative levels of androgen receptor activity in the cyclosporine-treated and vehicle-treated animals were similar. Following partial hepatectomy, a reduction in the activity of both sex hormone receptors in the hepatic cytosol was observed and was compatible with results described previously in normal animals. Unexpectedly the preoperative levels of ornithine decarboxylase (P less than 0.01) and thymidine kinase activity (P less than 0.01) were significantly greater in the rats treated with cyclosporine as compared to the vehicle treated controls. As expected, ornithine decarboxylase activity (at 6 hr) and thymidine kinase activity (at 24 hr) rose and peaked in response to a partial hepatectomy but were significantly greater (P less than 0.05) in the rats treated with cyclosporine as compared to the vehicle.(ABSTRACT TRUNCATED AT 250 WORDS)
据报道，肝脏中的雌激素和雄激素受体可调节肝脏对部分肝切除术的再生反应。此外，环孢素具有多种不良影响，可能是性激素活性改变的结果。为了评估这些问题，进行了以下实验。在部分肝切除术或假手术后，对用环孢素或橄榄油载体处理的大鼠肝脏中的细胞溶质雌激素和雄激素受体进行定量。评估鸟氨酸脱羧酶活性和胸苷激酶活性作为肝脏再生的指标。与载体处理的对照组相比，用环孢素处理的大鼠肝细胞质中雌激素受体活性的术前水平显著更高（P<0.01）。相反，环孢素处理组和载体处理组动物的雄激素受体活性术前水平相似。部分肝切除术后，观察到肝细胞质中两种性激素受体的活性均降低，这与先前在正常动物中描述的结果一致。出乎意料的是，与载体处理的对照组相比，用环孢素处理的大鼠术前鸟氨酸脱羧酶活性（P<0.01）和胸苷激酶活性（P<0.01）显著更高。正如预期的那样，鸟氨酸脱羧酶活性（6小时时）和胸苷激酶活性（24小时时）因部分肝切除术而升高并达到峰值，但与载体处理组相比，用环孢素处理的大鼠中这些活性显著更高（P<0.05）。（摘要截断于250字）
