单分子可视化研究光响应寡核苷酸的杂交和解离及其在 DNA 纳米结构中的可逆开关行为。
Single-molecule visualization of the hybridization and dissociation of photoresponsive oligonucleotides and their reversible switching behavior in a DNA nanostructure.
机构信息
Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501, Japan.
出版信息
Angew Chem Int Ed Engl. 2012 Oct 15;51(42):10518-22. doi: 10.1002/anie.201205247. Epub 2012 Sep 10.
PMID:22965475
Abstract
A framed photo of DNA: A pair of photoresponsive oligonucleotides containing azobenzene moieties was introduced into double-stranded DNA within the cavity of a DNA nanostructure (see scheme). The two dsDNAs, in contact at the center, were dissociated using UV irradiation and hybridized with visible light; this was directly observed using high-speed atomic force microscopy.
摘要
DNA 的框式照片：一对含有偶氮苯部分的光响应寡核苷酸被引入到 DNA 纳米结构腔体内的双链 DNA 中（见方案）。两个 dsDNA 在中心接触，使用紫外光照射进行解离，并通过可见光进行杂交；这可以通过高速原子力显微镜直接观察到。
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