Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
J Clin Microbiol. 2012 Nov;50(11):3805-8. doi: 10.1128/JCM.01469-12. Epub 2012 Sep 12.
Mixed infections with different Leishmania species could explain differences in the clinical courses of these infections. On identification of Leishmania parasites from Iranian patients with mucosal leishmaniasis (ML), a patient with both oral and nasal lesions was found to be concomitantly infected with Leishmania tropica and L. major. Mixed infection was identified by PCR amplification of Leishmania kinetoplast DNA on scraping of cytological smears and histopathological sections. L. major and L. tropica were isolated from the nasal and oral lesions, respectively. These species were also confirmed by immunohistochemistry. This seems to be the first reported case of concurrent ML infection with two Leishmania species. It indicates that, at least in this patient, previous infection with one of these Leishmania species did not protect against infection with the other. This result has important implications for the development of vaccines against leishmaniases and implies careful attention in the treatment of this infectious disease.
不同利什曼原虫物种的混合感染可能解释了这些感染的临床过程的差异。在鉴定伊朗黏膜利什曼病（ML）患者的利什曼原虫寄生虫时，发现一名口腔和鼻腔均有病变的患者同时感染了热带利什曼原虫和利什曼原虫。通过对细胞学涂片和组织病理学切片刮取物中的利什曼动基体 DNA 的 PCR 扩增来鉴定混合感染。从鼻腔和口腔病变中分别分离出利什曼原虫和利什曼原虫。免疫组织化学也证实了这两种物种。这似乎是首例同时感染两种利什曼原虫的 ML 报告病例。这表明，至少在该患者中，以前感染其中一种利什曼原虫并不能防止感染另一种利什曼原虫。这一结果对利什曼病疫苗的开发具有重要意义，并暗示在治疗这种传染病时应谨慎注意。
