Department of Chemistry, Northwestern University, Evanston, IL 60208, USA.
J Am Soc Mass Spectrom. 2012 Oct;23(10):1617-24. doi: 10.1007/s13361-012-0469-9. Epub 2012 Sep 14.
The general scope of a project to determine the protein molecules that comprise the cells within the human body is framed. By focusing on protein primary structure as expressed in specific cell types, this concept for a cell-based version of the Human Proteome Project (CB-HPP) is crafted in a manner analogous to the Human Genome Project while recognizing that cells provide a primary context in which to define a proteome. Several activities flow from this articulation of the HPP, which enables the definition of clear milestones and deliverables. The CB-HPP highlights major gaps in our knowledge regarding cell heterogeneity and protein isoforms, and calls for development of technology that is capable of defining all human cell types and their proteomes. The main activities will involve mapping and sorting cell types combined with the application of beyond the state-of-the art in protein mass spectrometry.
确定构成人体细胞的蛋白质分子的项目范围被确定。通过关注特定细胞类型中表达的蛋白质一级结构，这个基于细胞的人类蛋白质组计划（CB-HPP）的概念以类似于人类基因组计划的方式制定，同时认识到细胞提供了定义蛋白质组的主要背景。从 HPP 的阐述中引出了几个活动，这些活动使明确的里程碑和可交付成果得以定义。CB-HPP 突出了我们对细胞异质性和蛋白质同工型的知识的主要差距，并呼吁开发能够定义所有人类细胞类型及其蛋白质组的技术。主要活动将涉及映射和分类细胞类型，同时应用超越蛋白质质谱分析的最新技术。
