Nitric oxide (NO)-induced death of gram-negative bacteria from a light-controlled NO-releasing platform.

A NO-delivery platform has been fabricated from polydimethylsiloxane (PDMS) and Pluronic(®) F127 gel that contains the light-sensitive NO donor, [Mn(PaPy(3))(NO)]ClO(4). The material was assembled layer-by-layer. First, a thin PDMS membrane was cast. It was then layered with cold 25% (w/v) Pluronic(®) F127 gel mixed with [Mn(PaPy(3))(NO)]ClO(4). Finally, it was covered with a thick layer (nearly impermeable to NO) of PDMS (=polydimethoxysiloxane) to allow release of NO only from the thinner side upon exposure to light. Light-induced NO release from this layered material has been confirmed via NO-specific electrode and by a modified soft Griess-agar assay. Incorporation of ca. 8 mg/g of [Mn(PaPy(3))(NO)]ClO(4) in the Pluronic gel layer affords a material that drastically reduces the microbial loads of Acinetobacter baumannii and Pseudomonas aeruginosa via the antibiotic effects of the photoreleased NO. Application of this flexible layered NO-donating composite as bandage material has been proposed.