Norval M, Smith C
Ann Rheum Dis. 1979 Oct;38(5):456-62. doi: 10.1136/ard.38.5.456.
DNA and RNA were extracted from synovial membranes, synovial fibroblast cells, peripheral blood lymphocytes, and synovial fibroblast cells strains derived from patients with rheumatoid arthritis and other joint conditions. They were hybridised after immobilisation on nitrocellulose filters with iodinated viral nucleic acids extracted from measles, rubella virus, SV--40, and a retrovirus, RD--114. In addition, in situ-hybridisation was carried out on sections of synovial membranes by means of iodinated measles and rubella virus RNA. In no case did any hybridisation occur. Positive control systems included synovial fibroblast strains transformed with SV--40, LLC--MK2 cells chronically infected with rubella virus and RD cells infected with RD--114. It was concluded tht the synovial cells did not contain viral genomes of measles, rubella virus, SV--40, or RD--114, or at least at a level equivalent to the positive control cells.
从类风湿性关节炎患者和其他关节疾病患者的滑膜、滑膜成纤维细胞、外周血淋巴细胞以及滑膜成纤维细胞株中提取DNA和RNA。将它们固定在硝酸纤维素滤膜上后，与从麻疹、风疹病毒、SV - 40和一种逆转录病毒RD - 114中提取的碘化病毒核酸进行杂交。此外，通过碘化麻疹和风疹病毒RNA对滑膜切片进行原位杂交。在任何情况下均未发生杂交。阳性对照系统包括用SV - 40转化的滑膜成纤维细胞株、长期感染风疹病毒的LLC - MK2细胞以及感染RD - 114的RD细胞。得出的结论是，滑膜细胞不含有麻疹、风疹病毒、SV - 40或RD - 114的病毒基因组，或者至少其水平与阳性对照细胞相当。
