Chattopadhyay H, Chattopadhyay C, Natvig J B, Wiger D, Mellbye O J
Scand J Immunol. 1979;10(1):47-54. doi: 10.1111/j.1365-3083.1979.tb01333.x.
In the present study we describe a plaque-forming cell assay using erythrocytes coated with viral antigen, which detected anti-viral antibody-secreting cells against various viral antigens. These anti-viral antibody-secreting cell were studied in normal individuals with known viral infections and in rheumatoid arthritis patients. Rubella anti-viral antibody-secreting cells were present after induction in the peripheral blood of eight out of ten patients. No plaques were seen before induction. Synovial tissue of seven patients out of ten showed rubella-antigen-specific plaques before induction. In all three patients tested, the numbers of plaques increased after induction. The peripheral blood of only one patient showed plaque-forming cells against mumps virus and cytomegalovirus (CMV) antigen. No other patients showed any plaque against CMV, respiratory syncytial virus, mumps virus, measles virus, adenovirus, and varicella zoster virus antigens. The method appears to be promising in studying viral antibody-secreting cells in human immunopathology.
在本研究中，我们描述了一种使用包被病毒抗原的红细胞进行的空斑形成细胞试验，该试验可检测针对各种病毒抗原的抗病毒抗体分泌细胞。我们在已知病毒感染的正常个体和类风湿性关节炎患者中对这些抗病毒抗体分泌细胞进行了研究。十名患者中有八名在诱导后外周血中出现风疹抗病毒抗体分泌细胞。诱导前未观察到空斑。十名患者中有七名的滑膜组织在诱导前显示风疹抗原特异性空斑。在所有三名接受检测的患者中，诱导后空斑数量增加。仅一名患者的外周血显示出针对腮腺炎病毒和巨细胞病毒（CMV）抗原的空斑形成细胞。没有其他患者显示出针对CMV、呼吸道合胞病毒、腮腺炎病毒、麻疹病毒、腺病毒和水痘带状疱疹病毒抗原的任何空斑。该方法在研究人类免疫病理学中的病毒抗体分泌细胞方面似乎很有前景。
