Cockburn Andrew F, Dehlin Jonathan M, Ngan Tiffany, Crout Richard, Boskovic Goran, Denvir James, Primerano Donald, Plassman Brenda L, Wu Bei, Cuff Christopher F
Microbiology, Immunology & Cell Biology, School of Medicine, Robert C, Byrd Health Sciences Center, West Virginia University, P,O, Box 4622, Morgantown, WV, 26506-4622, USA.
Investig Genet. 2012 Sep 21;3(1):19. doi: 10.1186/2041-2223-3-19.
To investigate the potential association between oral health and cognitive function, a pilot study was conducted to evaluate high throughput DNA sequencing of the V3 region of the 16S ribosomal RNA gene for determining the relative abundance of bacterial taxa in subgingival plaque from older adults with or without dementia.
Subgingival plaque samples were obtained from ten individuals at least 70 years old who participated in a study to assess oral health and cognitive function. DNA was isolated from the samples and a gene segment from the V3 portion of the 16S bacterial ribosomal RNA gene was amplified and sequenced using an Illumina HiSeq1000 DNA sequencer. Bacterial populations found in the subgingival plaque were identified and assessed with respect to the cognitive status and oral health of the participants who provided the samples.
More than two million high quality DNA sequences were obtained from each sample. Individuals differed greatly in the mix of phylotypes, but different sites from different subgingival depths in the same subject were usually similar. No consistent differences were observed in this small sample between subjects separated by levels of oral health, sex, or age; however a consistently higher level of Fusobacteriaceae and a generally lower level of Prevotellaceae was seen in subjects without dementia, although the difference did not reach statistical significance, possibly because of the small sample size.
The results from this pilot study provide suggestive evidence that alterations in the subgingival microbiome are associated with changes in cognitive function, and provide support for an expanded analysis of the role of the oral microbiome in dementia.
为了研究口腔健康与认知功能之间的潜在关联，开展了一项初步研究，以评估16S核糖体RNA基因V3区域的高通量DNA测序，用于确定患有或未患有痴呆症的老年人龈下菌斑中细菌类群的相对丰度。
从10名至少70岁的个体中获取龈下菌斑样本，这些个体参与了一项评估口腔健康和认知功能的研究。从样本中分离出DNA，并使用Illumina HiSeq1000 DNA测序仪对16S细菌核糖体RNA基因V3部分的基因片段进行扩增和测序。根据提供样本的参与者的认知状态和口腔健康情况，对龈下菌斑中发现的细菌种群进行鉴定和评估。
每个样本获得了超过两百万条高质量的DNA序列。个体之间的系统发育型组合差异很大，但同一受试者不同龈下深度的不同部位通常相似。在这个小样本中，按口腔健康水平、性别或年龄划分的受试者之间未观察到一致差异；然而，在未患痴呆症的受试者中，梭杆菌科的水平始终较高，普雷沃氏菌科的水平普遍较低，尽管差异未达到统计学意义，可能是由于样本量较小。
这项初步研究的结果提供了提示性证据，表明龈下微生物群的改变与认知功能的变化有关，并为扩大分析口腔微生物群在痴呆症中的作用提供了支持。
