Department of Bacteriology and Virology, Faculty of Pharmacy, University of Cocody, Abidjan, Côte d'Ivoire.
PLoS One. 2012;7(9):e45363. doi: 10.1371/journal.pone.0045363. Epub 2012 Sep 18.
Genotyping methods are useful tools to provide information on tuberculosis epidemic. They can allow a better response from health authorities and the implementation of measures for tuberculosis control. This study aimed to identify the main lineages and clades of Mycobacterium tuberculosis complex strains circulating in Côte d'Ivoire.
METHODS/MAIN FINDINGS: Strains isolated from sputum samples of patients ongoing retreatment from all the country were characterized by spoligotyping and by MIRU-VNTR. Profiles obtained by spoligotyping were first compared to the SITVIT/SpolDB4 database for family assignment. Of 194 strains analysed, 146 (75.3%) belonged to the T lineage. The most predominant spoligotype was the shared international type 53 with 135 strains (69.6%). In contrast with neighbouring countries, LAM (11 strains, 5.7%) and H (9 strains 4.6%) lineages were slightly represented. Only 3 Beijing strains (1.5%) and 4 strains of Mycobacterium africanum (2%) were found. Analysis of the results obtained with MIRU-VNTR revealed also a high level of clustering.
CONCLUSION/SIGNIFICANCE: The population of Mycobacterium tuberculosis complex strains among retreatment cases in Côte d'Ivoire exhibits a low diversity, allowing to assume recent transmission and locally based infection.
基因分型方法是提供结核病流行信息的有用工具。它们可以使卫生当局更好地做出反应，并实施结核病控制措施。本研究旨在确定科特迪瓦流行的结核分枝杆菌复合菌株的主要谱系和分支。
方法/主要发现：对来自全国各地正在进行复治的患者的痰液样本中分离出的菌株进行 spoligotyping 和 MIRU-VNTR 分析。首先将 spoligotyping 获得的谱图与 SITVIT/SpolDB4 数据库进行比较，以确定家族归属。在分析的 194 株菌株中，146 株（75.3%）属于 T 谱系。最主要的 spoligotype 是共有国际型 53，有 135 株（69.6%）。与邻国相比，LAM（11 株，5.7%）和 H（9 株，4.6%）谱系的菌株略有代表。仅发现 3 株北京株（1.5%）和 4 株非洲分枝杆菌（2%）。MIRU-VNTR 分析结果也显示出高度的聚类。
结论/意义：科特迪瓦复治病例中结核分枝杆菌复合菌株的种群多样性较低，可假定为近期传播和本地感染。
