Department of Natural Product Chemistry, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, People's Republic of China.
Phytochemistry. 2013 Jan;85:185-93. doi: 10.1016/j.phytochem.2012.08.015. Epub 2012 Oct 1.
HPLC-PDA-MS and TLC analysis were used to look for minor cytotoxic chlorinated valepotriates from whole plants of Valeriana jatamansi (syn. Valeriana wallichii DC.). This resulted in isolation of 15 chlorinated valepotriates, designated as chlorovaltrates A-O, together with six known analogues, (1S,3R,5R,7S,8S,9S)-3,8-epoxy-1,5-dihydroxyvalechlorine, volvaltrate B, chlorovaltrate, rupesin B, (1S,3R,5R,7S,8S,9S)-3,8-epoxy-1-O-ethyl-5-hydroxyvalechlorine, and (1R,3R,5R,7S,8S,9S)-3,8-epoxy-1-O-ethyl-5-hydroxyvalechlorine. Their structures were elucidated by spectroscopic methods including homo- and heteronuclear two-dimensional NMR experiments. Chlorovaltrates K-N, chlorovaltrate and rupesin B showed moderate cytotoxicity against lung adenocarcinoma (A 549), metastatic prostate cancer (PC-3M), colon cancer (HCT-8) and hepatoma (Bel 7402) cell lines with IC₅₀ values of 0.89-9.76 μM.
采用 HPLC-PDA-MS 和 TLC 分析方法，从缬草（缬草）全植物中寻找微量细胞毒性的氯化缬草酯。这导致了 15 种氯化缬草酯的分离，命名为氯缬草 A-O，以及六种已知类似物，（1S,3R,5R,7S,8S,9S）-3,8-环氧-1,5-二羟基缬草氯，缬草 B，氯缬草酯，鲁斯宾 B，（1S,3R,5R,7S,8S,9S）-3,8-环氧-1-O-乙基-5-羟基缬草氯，和（1R,3R,5R,7S,8S,9S）-3,8-环氧-1-O-乙基-5-羟基缬草氯。通过包括同核和异核二维 NMR 实验在内的光谱方法阐明了它们的结构。氯缬草酯 K-N、氯缬草酯和鲁斯宾 B 对肺腺癌细胞（A549）、转移性前列腺癌细胞（PC-3M）、结肠癌细胞（HCT-8）和肝癌细胞（Bel 7402）表现出中等细胞毒性，IC50 值为 0.89-9.76 μM。
