Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA.
Opt Lett. 2012 Sep 15;37(18):3882-4. doi: 10.1364/ol.37.003882.
Organs such as the heart and brain possess intricate fiber structures that are best characterized with three-dimensional imaging. For instance, diffusion-based, magnetic resonance tractography (MRT) enables studies of connectivity and remodeling during development and disease macroscopically on the millimeter scale. Here we present complementary, high-resolution microscopic optical coherence imaging and analysis methods that, when used in conjunction with clearing techniques, can characterize fiber architecture in intact organs at tissue depths exceeding 1 mm. We anticipate that these techniques can be used to study fiber architecture in situ at microscopic scales not currently accessible to diffusion magentic resonance (MR), and thus, to validate and complement macroscopic structural imaging techniques. Moreover, as these techniques use intrinsic signals and do not require tissue slicing and staining, they can be used for high-throughput, nondestructive evaluation of fiber architecture across large tissue volumes.
心脏和大脑等器官具有复杂的纤维结构，最好通过三维成像来进行描述。例如，基于扩散的磁共振纤维束示踪（MRT）可以在毫米尺度上宏观地研究发育和疾病过程中的连通性和重塑。在这里，我们提出了互补的高分辨率微观光学相干成像和分析方法，当与透明化技术结合使用时，可以在超过 1 毫米的组织深度处对完整器官中的纤维结构进行特征描述。我们预计这些技术可以用于在目前扩散磁共振（MR）无法到达的微观尺度上原位研究纤维结构，从而验证和补充宏观结构成像技术。此外，由于这些技术使用固有信号，并且不需要组织切片和染色，因此它们可以用于高通量、无损地评估大片组织体积中的纤维结构。
