CBS-KNAW Fungal Biodiversity Centre, Utrecht, the Netherlands.
J Clin Microbiol. 2013 Jan;51(1):22-30. doi: 10.1128/JCM.02147-12. Epub 2012 Oct 17.
In recent years, Geosmithia argillacea has been increasingly reported in humans and animals and can be considered an emerging pathogen. The taxonomy of Geosmithia was recently studied, and Geosmithia argillacea and related species were transferred to the new genus Rasamsonia. The diversity among a set of Rasamsonia argillacea strains, including 28 clinical strains, was studied, and antifungal susceptibility profiles were generated. Data obtained from morphological studies and from phylogenetic analyses of internal transcribed spacer (ITS) and partial β-tubulin and calmodulin sequences revealed the presence of four species in the Rasamsonia argillacea complex, two of which are newly described here: R. piperina sp. nov. and R. aegroticola sp. nov. In contrast to other related genera, all Rasamsonia species can be identified with ITS sequences. A retrospective identification was performed on recently reported clinical isolates from animal or human patients. Susceptibility tests showed that the antifungal susceptibility profiles of the four members of the R. argillacea complex are similar, and caspofungin showed significant activity in vitro, followed by amphotericin B and posaconazole. Voriconazole was the least active of the antifungals tested. The phenotypically similar species R. brevistipitata and R. cylindrospora had different antifungal susceptibility profiles, and this indicates that correct species identification is important to help guide appropriate antifungal therapy.
近年来，粘帚霉属（Geosmithia）在人和动物中越来越多地被报道，可以被认为是一种新兴的病原体。最近对粘帚霉属的分类进行了研究，将粘球帚霉（Geosmithia argillacea）和相关物种转移到新属 Rasamsonia 中。对一组粘帚霉属菌株（包括 28 株临床分离株）的多样性进行了研究，并生成了抗真菌药敏谱。形态学研究和内部转录间隔区（ITS）和部分β-微管蛋白和钙调蛋白序列的系统发育分析获得的数据表明，在 Rasamsonia argillacea 复合体中存在四个物种，其中两个是新描述的：R. piperina sp. nov. 和 R. aegroticola sp. nov. 与其他相关属不同，所有 Rasamsonia 物种都可以用 ITS 序列来鉴定。对最近从动物或人类患者中报告的临床分离株进行了回顾性鉴定。药敏试验表明，R. argillacea 复合体的四个成员的抗真菌药敏谱相似，卡泊芬净在体外具有显著的活性，其次是两性霉素 B 和泊沙康唑。伏立康唑是测试的抗真菌药物中活性最低的。表型相似的 R. brevistipitata 和 R. cylindrospora 具有不同的抗真菌药敏谱，这表明正确的物种鉴定对于指导适当的抗真菌治疗非常重要。
