Ribeiro Caroline Sousa, Kuteken Fernanda Akemi, Hirata Júnior Raphael, Scelza Miriam F Zaccaro
Department of Endodontics, Fluminense Federal University, Niterói, RJ, Brazil.
J Appl Oral Sci. 2006 Oct;14(5):330-3. doi: 10.1590/s1678-77572006000500006.
The present study aimed to evaluate and compare the antimicrobial effect of MTA Dentsply, MTA Angelus, Calcium Hydroxide and Portland cement. Four reference bacterial strains were used: Pseudomonas aeruginosa, Escherichia coli, Bacteroides fragilis, and Enterococcus faecalis. Plates containing Mueller-Hinton agar supplemented with 5% sheep blood, hemin, and menadione were inoculated with the bacterial suspensions. Subsequently, wells were prepared and immediately filled with materials and incubated at 37 degrees C for 48 hours under anaerobic conditions, except P. aeruginosa. The diameters of inhibition zones were measured, and data analyzed using ANOVA and the Tukey test with 1% level of significance. MTA Dentsply, MTA Angelus and Portland cement inhibited the growth of P. aeruginosa. Calcium Hydroxide was effective against P. aeruginosa and B. fragillis. Under anaerobic conditions, which may hamper the formation of reactive oxygen species, the materials failed to inhibit E. faecalis, and E. coli.
本研究旨在评估和比较登士柏MTA、安格勒斯MTA、氢氧化钙和波特兰水泥的抗菌效果。使用了四种参考细菌菌株：铜绿假单胞菌、大肠杆菌、脆弱拟杆菌和粪肠球菌。将含有补充了5%羊血、血红素和甲萘醌的穆勒-欣顿琼脂的平板接种细菌悬液。随后，制备孔并立即填充材料，除铜绿假单胞菌外，在厌氧条件下于37℃孵育48小时。测量抑菌圈直径，并使用方差分析和显著性水平为1%的Tukey检验分析数据。登士柏MTA、安格勒斯MTA和波特兰水泥抑制了铜绿假单胞菌的生长。氢氧化钙对铜绿假单胞菌和脆弱拟杆菌有效。在可能阻碍活性氧形成的厌氧条件下，这些材料未能抑制粪肠球菌和大肠杆菌。
