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一种基于基因种水平的临床和环境气单胞菌菌株快速 MALDI-TOF MS 鉴定数据库。

A rapid MALDI-TOF MS identification database at genospecies level for clinical and environmental Aeromonas strains.

机构信息

Institute of Microbiology, Bellinzona, Switzerland.

出版信息

PLoS One. 2012;7(10):e48441. doi: 10.1371/journal.pone.0048441. Epub 2012 Oct 31.

Abstract

The genus Aeromonas has undergone a number of taxonomic and nomenclature revisions over the past 20 years, and new (sub)species and biogroups are continuously described. Standard identification methods such as biochemical characterization have deficiencies and do not allow clarification of the taxonomic position. This report describes the development of a matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS) identification database for a rapid identification of clinical and environmental Aeromonas isolates.

摘要

过去 20 年来,气单胞菌属经历了多次分类和命名修订,并且不断描述新的(亚)种和生物群。标准的鉴定方法,如生化特征,存在缺陷,无法明确分类地位。本报告描述了基质辅助激光解吸/电离-飞行时间质谱(MALDI-TOF MS)鉴定数据库的开发,用于快速鉴定临床和环境中气单胞菌分离株。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2419/3485216/6d4d03fce067/pone.0048441.g001.jpg

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