壳聚糖生物分子对鸡三毛滴虫的新型体外作用效率
Novel in Vitro Efficiency of Chitosan Biomolecule against Trichomonas gallinae.
作者信息
Tavassoli M, Imani A, Tajik H, Moradi M, Pourseyed Sh
机构信息
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
出版信息
Iran J Parasitol. 2012;7(1):92-6.
BACKGROUND
Development of new natural agents for parasitic diseases treatment has unexpectedly increased to overcome effectively against emergence and re-emergence of parasitic diseases, the appearance of drug resistant organisms and toxic side effects of current agents. The aim of the study was to evaluate antiprotozoal activities of chitosan biomolecule on trophozoites of Trichomonas gallinae.
METHODS
The antitrichomonal activity of various low molecular weight chitosan concentrations including 125, 250, 500 and 1250 µg ml(-1) against T. gallinae trophozoites cultured in trypticase-yeast extract-maltose medium supplemented with heat-inactivated cold horse serum was evaluated in vitro. Samples containing medium without chitosan were also assayed as controls.
RESULTS
The mortality rates at 0, 3 and 6 h post treatment with all concentrations were significantly different from control group (P<0.05). Treated trophozoites showed more susceptibility to the highest concentration reaching mortality rate of 100% at 3h post inoculation. However, at this time, results for 125, 250 and 500 µg ml(-1) were 93%, 95% and 96.7%, respectively.
CONCLUSION
The results demonstrate that the application of chitosan biomolecule is a promising option for treatment of trichomoniasis in pigeons.
背景
为有效应对寄生虫病的出现与再出现、耐药生物体的出现以及现有药物的毒副作用，用于治疗寄生虫病的新型天然药物的研发意外增加。本研究的目的是评估壳聚糖生物分子对鸡三毛滴虫滋养体的抗原生动物活性。
方法
在补充有热灭活冷马血清的胰蛋白酶-酵母提取物-麦芽糖培养基中培养鸡三毛滴虫滋养体，体外评估包括125、250、500和1250µg/ml在内的各种低分子量壳聚糖浓度对其的抗滴虫活性。不含壳聚糖的培养基样本也作为对照进行检测。
结果
所有浓度处理后0、3和6小时的死亡率与对照组有显著差异（P<0.05）。处理后的滋养体对最高浓度更敏感，接种后3小时死亡率达到100%。然而，此时125、250和500µg/ml的结果分别为93%、95%和96.7%。
结论
结果表明壳聚糖生物分子的应用是治疗鸽毛滴虫病的一个有前景的选择。
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