Mackenstedt U, Gauer M, Mehlhorn H, Schein E, Hauschild S
Lehrstuhl für Spezielle Zoologie und Parasitologie, Ruhr-Universität Bochum, Federal Republic of Germany.
Parasitol Res. 1990;76(3):199-206. doi: 10.1007/BF00930815.
The DNA content of the developmental stages within the life cycle of Babesia divergens was measured by means of fluorescence microscopy using the DNA-specific bisbenzimide Hoechst 33258. By comparing the mean relative fluorescence intensities, the assumption of sexual reproduction in the gut of the tick vector (Ixodes ricinus) was confirmed. However, no proof of a pre- or postzygotic meiosis was found.
利用DNA特异性双苯甲酰亚胺Hoechst 33258,通过荧光显微镜测量了分歧巴贝斯虫生命周期内各发育阶段的DNA含量。通过比较平均相对荧光强度,证实了蜱传播媒介(蓖麻硬蜱)肠道内存在有性繁殖的假设。然而,未发现合子前或合子后减数分裂的证据。