Straube W L, O'Brien M, Davis K, Colwell R R
Department of Microbiology, University of Maryland, College Park 20742.
Appl Environ Microbiol. 1990 Mar;56(3):812-4. doi: 10.1128/aem.56.3.812-814.1990.
Barophilic bacteria are microorganisms that grow preferentially (facultative barophiles) or exclusively (obligate barophiles) under elevated hydrostatic pressure. Barophilic bacteria have been isolated from a variety of deep-sea environments. Attempts to characterize these organisms have been hampered by a lack of appropriate methodologies. A colorimetric method for the detection of 19 constitutively expressed enzymes under in situ conditions of pressure and temperature has been devised, using a simple modification of the commercially available API ZYME enzyme assay kit. By using this method, enzyme profiles of 11 barophilic isolates, including an obligate barophile, were determined. Nine of the 10 facultatively barophilic isolates examined exhibited a change of phenotype in at least one enzyme reaction when tested at 1 atm (1 atm = 101.29 kPa), compared with results obtained under in situ pressure. The assay is simple and rapid and allows for direct determination of enzyme activity under conditions of high pressure and low temperature.
嗜压细菌是一类在升高的静水压力下优先生长（兼性嗜压菌）或仅能生长（专性嗜压菌）的微生物。嗜压细菌已从各种深海环境中分离出来。由于缺乏合适的方法，对这些生物进行表征的尝试受到了阻碍。通过对市售的API ZYME酶检测试剂盒进行简单修改，设计了一种比色法，用于在压力和温度的原位条件下检测19种组成型表达的酶。通过使用这种方法，确定了11株嗜压分离株的酶谱，其中包括一株专性嗜压菌。与原位压力下获得的结果相比，在1个大气压（1个大气压 = 101.29千帕）下测试时，所检测的10株兼性嗜压分离株中有9株在至少一种酶反应中表现出表型变化。该检测方法简单快速，能够在高压和低温条件下直接测定酶活性。
