Li L, Kato C, Nogi Y, Horikoshi K
DEEPSTAR Group, Japan Marine and Science and Technology Center (JAMSTEC), Yokosuka, Japan.
FEMS Microbiol Lett. 1998 Feb 15;159(2):159-66. doi: 10.1111/j.1574-6968.1998.tb12855.x.
DNA regions corresponding to portions of two different pressure-regulated operons previously identified in two deep-sea barophilic bacteria were separately PCR amplified from a variety of deep-sea microorganisms and sequenced. With the two sets of primers employed, amplification was particularly successful from the more barophilic bacteria examined. 16S rRNA sequence analysis revealed that these bacteria are all phylogenetically related and belong in a sub-branch of the genus Shewanella containing only the deep-sea Shewanella barophilic bacteria. We define this sub-branch as the 'Shewanella barophile branch' containing at least two different species. Our results suggest that the DNA sequences of the pressure-regulated operons can be regarded as marker sequences to identify the Shewanella barophilic strains.
从多种深海微生物中分别PCR扩增出与先前在两种深海嗜压细菌中鉴定出的两个不同压力调节操纵子部分相对应的DNA区域，并进行测序。使用这两组引物，从所检测的嗜压性更强的细菌中扩增特别成功。16S rRNA序列分析表明，这些细菌在系统发育上均相关，属于希瓦氏菌属的一个亚分支，该亚分支仅包含深海嗜压希瓦氏菌。我们将这个亚分支定义为包含至少两个不同物种的“嗜压希瓦氏菌分支”。我们的结果表明，压力调节操纵子的DNA序列可被视为鉴定嗜压希瓦氏菌菌株的标记序列。
