Department of Biomedical Engineering, College of Engineering, University of Michigan, 2800 Plymouth Road, Ann Arbor, Michigan 48109, United States.
Nano Lett. 2012 Dec 12;12(12):6480-4. doi: 10.1021/nl304063f. Epub 2012 Nov 28.
This paper describes a novel nanofluidic phenomenon where untethered DNA and chromatin are linearized by rapidly narrowing an elastomeric nanochannel filled with solutions of the biopolymers. This nanoscale squeezing procedure generates hydrodynamic flows while also confining the biopolymers into smaller and smaller volumes. The unique features of this technique enable full linearization then trapping of biopolymers such as DNA. The versatility of the method is also demonstrated by analysis of chromatin stretchability and mapping of histone states using single strands of chromatin.
本文描述了一种新颖的纳米流变体现象，即通过快速缩小填充有生物聚合物溶液的弹性纳米通道来线性化无束缚的 DNA 和染色质。这种纳米级挤压过程会产生流体动力流，同时也将生物聚合物压缩到越来越小的体积中。该技术的独特之处在于能够完全线性化并捕获生物聚合物，如 DNA。该方法的多功能性还通过使用染色质单链分析染色质的可拉伸性和描绘组蛋白状态得到了证明。
