通过靶向亚硫酸氢盐测序分析癌症甲基组。
Analyzing the cancer methylome through targeted bisulfite sequencing.
机构信息
GHSU Cancer Center, Department of Biochemistry and Molecular Biology, Georgia Health Sciences University, Augusta, GA 30912, USA.
出版信息
Cancer Lett. 2013 Nov 1;340(2):171-8. doi: 10.1016/j.canlet.2012.10.040. Epub 2012 Nov 28.
Abstract
Bisulfite conversion of genomic DNA combined with next-generation sequencing (NGS) has become a very effective approach for mapping the whole-genome and sub-genome wide DNA methylation landscapes. However, whole methylome shotgun bisulfite sequencing is still expensive and not suitable for analyzing large numbers of human cancer specimens. Recent advances in the development of targeted bisulfite sequencing approaches offer several attractive alternatives. The characteristics and applications of these methods are discussed in this review article. In addition, the bioinformatic tools that can be used for sequence capture probe design as well as downstream sequence analyses are also addressed.
摘要
亚硫酸氢盐转化基因组 DNA 与下一代测序（NGS）相结合，已成为绘制全基因组和亚基因组范围 DNA 甲基化图谱的非常有效的方法。然而，全甲基组鸟枪法亚硫酸氢盐测序仍然昂贵，不适合分析大量人类癌症标本。靶向亚硫酸氢盐测序方法的最新进展提供了几种有吸引力的替代方法。本文综述了这些方法的特点和应用。此外，还讨论了可用于序列捕获探针设计以及下游序列分析的生物信息学工具。
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