Genuis Stephen J, Beesoon Sanjay, Lobo Rebecca A, Birkholz Detlef
Faculty of Medicine, University of Alberta, 2935-66 Street, Edmonton, AB T6K 4C1, Canada.
ScientificWorldJournal. 2012;2012:615068. doi: 10.1100/2012/615068. Epub 2012 Oct 31.
Individual members of the phthalate family of chemical compounds are components of innumerable everyday consumer products, resulting in a high exposure scenario for some individuals and population groups. Multiple epidemiological studies have demonstrated statistically significant exposure-disease relationships involving phthalates and toxicological studies have shown estrogenic effects in vitro. Data is lacking in the medical literature, however, on effective means to facilitate phthalate excretion.
Blood, urine, and sweat were collected from 20 individuals (10 healthy participants and 10 participants with assorted health problems) and analyzed for parent phthalate compounds as well as phthalate metabolites using high performance liquid chromatography-tandem mass spectrometry.
Some parent phthalates as well as their metabolites were excreted into sweat. All patients had MEHP (mono(2-ethylhexyl) phthalate) in their blood, sweat, and urine samples, suggesting widespread phthalate exposure. In several individuals, DEHP (di (2-ethylhexl) phthalate) was found in sweat but not in serum, suggesting the possibility of phthalate retention and bioaccumulation. On average, MEHP concentration in sweat was more than twice as high as urine levels.
Induced perspiration may be useful to facilitate elimination of some potentially toxic phthalate compounds including DEHP and MEHP. Sweat analysis may be helpful in establishing the existence of accrued DEHP in the human body.
邻苯二甲酸酯类化合物中的个别成分是无数日常消费品的组成部分，这使得一些个体和人群面临高暴露风险。多项流行病学研究已证明邻苯二甲酸酯与疾病之间存在具有统计学意义的暴露-疾病关系，毒理学研究也显示了其在体外的雌激素效应。然而，医学文献中缺乏关于促进邻苯二甲酸酯排泄的有效方法的数据。
从20名个体（10名健康参与者和10名有各种健康问题的参与者）采集血液、尿液和汗液，使用高效液相色谱-串联质谱法分析母体邻苯二甲酸酯化合物及其代谢物。
一些母体邻苯二甲酸酯及其代谢物被排泄到汗液中。所有患者的血液、汗液和尿液样本中均含有MEHP（邻苯二甲酸单（2-乙基己基）酯），这表明邻苯二甲酸酯暴露广泛。在一些个体中，汗液中发现了DEHP（邻苯二甲酸二（2-乙基己基）酯），但血清中未发现，这表明邻苯二甲酸酯可能存在滞留和生物蓄积现象。平均而言，汗液中MEHP的浓度是尿液中浓度的两倍多。
诱导排汗可能有助于促进某些潜在有毒邻苯二甲酸酯化合物（包括DEHP和MEHP）的排泄。汗液分析可能有助于确定人体中是否存在累积的DEHP。
