Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.
Am J Trop Med Hyg. 2013 Feb;88(2):301-8. doi: 10.4269/ajtmh.2012.12-0209. Epub 2012 Dec 18.
The human landing catch (HLC) has long been the gold standard for estimating malaria transmission by mosquitoes, but has come under scrutiny because of ethical concerns of exposing collectors to infectious bites. We estimated the incidence of Plasmodium falciparum malaria infection in a cohort of 152 persons conducting HLCs and compared it with that of 147 non-collectors in western Kenya. Participants were presumptively cleared of malaria with Coartem™ (artemether-lumefantrine) and tested for malaria every 2 weeks for 12 weeks. The HLC collections were conducted four nights per week for six weeks. Collectors were provided chemoprophylaxis with Malarone™ (atovaquone-proguanil) during the six weeks of HLC activities and one week after HLC activities were completed. The incidence of malaria was 96.6% lower in collectors than in non-collectors (hazard ratio = 0.034, P < 0.0001). Therefore, with proper prophylaxis, concern about increased risk of malaria among collectors should not be an impediment to conducting HLC studies.
人体捕获（HLC）一直是估计蚊子传播疟疾的黄金标准，但由于让收集者接触传染性叮咬会引起伦理问题，因此受到了质疑。我们在肯尼亚西部的 152 名进行 HLC 的人群中估计了恶性疟原虫疟疾感染的发病率，并将其与 147 名非收集者进行了比较。参与者用 Coartem（青蒿琥酯-甲氟喹）进行了疟疾的推定清除，并在 12 周内每 2 周进行一次疟疾检测。HLC 收集每周进行四天，持续六周。在 HLC 活动的六周内以及 HLC 活动完成后的一周内，为收集者提供 Malarone（阿托伐醌-丙氨嘧啶）化学预防。收集者疟疾的发病率比非收集者低 96.6%（风险比=0.034，P<0.0001）。因此，只要进行适当的预防，就不必担心收集者疟疾风险增加会成为进行 HLC 研究的障碍。
