Institute of Condensed Matter and Nanosciences, Bio- & Soft Matter Division, Université catholique de Louvain, Croix du Sud 1, Louvain-la-Neuve, Belgium.
Biomacromolecules. 2013 Feb 11;14(2):520-8. doi: 10.1021/bm301774a. Epub 2013 Jan 15.
Photo-cross-linkable polyelectrolyte films, whose nanomechanical properties can be varied under UV light illumination, were prepared from poly(l-lysine) (PLL) and a hyaluronan derivative modified with photoreactive vinylbenzyl groups (HAVB). The adhesion and the growth of two model bacteria, namely Escherichia coli and Lactococcus lactis , were studied on non-cross-linked and cross-linked films to investigate how the film stiffness influences the bacterial behavior. While the Gram positive L. lactis was shown to grow slowly on both films, independently of their rigidity, the Gram negative E. coli exhibited a more rapid growth on non-cross-linked softer films compared to the stiffer ones. Experiments performed on photopatterned films showing both soft and stiff regions, confirmed a faster development of E. coli colonies on softer regions. Interestingly, this behavior is opposite to the one reported before for mammalian cells. Therefore, the photo-cross-linked (PLL/HAVB) films are interesting coatings for tissue engineering since they promote the growth of mammalian cells while limiting the bacterial colonization.
光交联聚电解质膜，其纳米力学性能可在紫外光照射下发生变化，由聚（L-赖氨酸）（PLL）和经光反应性乙烯基苄基基团修饰的透明质酸衍生物（HAVB）制备而成。在非交联和交联膜上研究了两种模型细菌，即大肠杆菌和乳酸乳球菌的黏附和生长，以研究膜的刚度如何影响细菌的行为。虽然革兰氏阳性的 L. lactis 在两种膜上均缓慢生长，而与膜的刚性无关，但革兰氏阴性的 E. coli 在非交联较软的膜上的生长速度比在较硬的膜上更快。在显示软区和硬区的光图案化膜上进行的实验证实，在较软的区域，E. coli 菌落的发育速度更快。有趣的是，这种行为与之前报道的哺乳动物细胞的行为相反。因此，光交联（PLL/HAVB）膜是组织工程中有趣的涂层，因为它们促进哺乳动物细胞的生长，同时限制细菌的定植。
