Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK.
Phys Chem Chem Phys. 2013 Apr 21;15(15):5312-28. doi: 10.1039/c2cp43859c.
The ability to develop new and sensitive methods of biomolecule detection is crucial to the advancement of pre-clinical disease diagnosis and effective patient specific treatment. Surface enhanced Raman scattering (SERS) is an optical spectroscopy amenable to this goal, as it is capable of extremely sensitive biomolecule detection and multiplexed analysis. This perspective highlights where SERS has been successfully used to detect target biomolecules, specifically DNA and proteins, and where in vivo analysis has been successfully utilised. The future of SERS development is discussed and emphasis is placed on the steps required to transport this novel technique from the research laboratory to a clinical setting for medical diagnostics.
开发新的和敏感的生物分子检测方法的能力对于临床前疾病诊断和有效的患者特异性治疗的进展至关重要。表面增强拉曼散射（SERS）是一种可实现此目标的光学光谱技术，因为它能够进行极其敏感的生物分子检测和多重分析。本观点重点介绍了 SERS 已成功用于检测目标生物分子（特别是 DNA 和蛋白质）的应用，并介绍了成功进行体内分析的情况。讨论了 SERS 的未来发展，并强调了将这项新技术从研究实验室转移到临床用于医学诊断所需的步骤。
